GKN1 Human

Gastrokine 1 Human Recombinant
Cat. No.
BT10285
Source
E.coli.
Synonyms
BRICHOS domain containing 1, 18 kDa antrum mucosa protein, gastrokine 1, Protein CA11, AMP-18, BRICD1, foveolin, FOV.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GKN1 Human Recombinant produced in E. coli is a single polypeptide chain containing 223 amino acids (1-199) and having a molecular mass of 24.5 kDa.
GKN1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Gastrokine-1 (GKN1), a member of the gastrokine family, exhibits mitogenic properties and contributes to maintaining the integrity of the gastric mucosal epithelium. In human gastric cancer tissue, GKN1 expression is reduced compared to normal gastric mucosa. While GKN1 is found in the stomach, it is absent in cancer tissue and gastric cancer cell lines.
Description
Recombinant human GKN1, produced in E. coli, is a single polypeptide chain comprising 223 amino acids (residues 1-199) with a molecular weight of 24.5 kDa. It features a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The GKN1 solution (0.25 mg/mL) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85% as assessed by SDS-PAGE analysis.
Synonyms
BRICHOS domain containing 1, 18 kDa antrum mucosa protein, gastrokine 1, Protein CA11, AMP-18, BRICD1, foveolin, FOV.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSH MLAYSS VHCFREDKMK FTIVFAGLLG VFLAPALANY NINVNDDNNN AGSGQQSVSV NNEHNVANVD NNNGWDSWNS IWDYGNGFAA TRLFQKKTCI VHKMNKEVMP SIQSLDALVK EKKLQGKGPG GPPPKGLMYS VNPNKVDDLS KFGKNIANMC RGIPTYMAEE MQEASLFFYS GTCYTTSVLW IVDISFCGDT VEN.

Product Science Overview

Introduction

Gastrokine 1 (GKN1) is a stomach-specific protein that plays a crucial role in maintaining the integrity and homeostasis of the gastric mucosal epithelium. It is produced by gastric mucus-secreting cells and is stored in cytoplasmic granules before being secreted with mucus onto the apical cell surface .

Structure and Expression

GKN1 is composed of 185 amino acid residues and has a molecular weight of approximately 18 kDa . It is primarily expressed in the mucous-secreting pyloric atrial epithelium . The recombinant form of GKN1 is often expressed in Escherichia coli and is used in various research applications, including SDS-PAGE and mass spectrometry .

Biological Functions

GKN1 has several important biological functions:

  1. Mucosal Integrity: GKN1 helps maintain the integrity of the gastric mucosal epithelium by promoting epithelial proliferation and migration .
  2. Cell Proliferation and Differentiation: It regulates cell proliferation and differentiation, which are essential processes for maintaining mucosal homeostasis .
  3. Tumor Suppression: GKN1 acts as a tumor suppressor, and its inactivation is one of the leading causes of gastric cancer . It inhibits cell proliferation and induces apoptosis in gastric cancer cells .
Clinical Significance

GKN1 has emerged as a potential biomarker for gastric cancer. Studies have shown that GKN1 is absent in gastric cancer tissues and derived cell lines, making it a valuable target for diagnosis and treatment . Additionally, exosomes carrying GKN1 have been found to inhibit gastric tumorigenesis, further supporting its role in cancer therapy .

Recombinant GKN1

Recombinant human GKN1 is a full-length protein expressed in Escherichia coli with a purity of over 85% . It is used in various research applications to study its biological properties and potential therapeutic uses. The recombinant protein is biologically active and may be involved in maintaining the integrity of the gastric mucosal epithelium .

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THE BIOTEK
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