GIPC2 Antibody

GIPC PDZ Domain Member 2, Mouse Anti Human
Cat. No.
BT18730
Source
Synonyms
GIPC PDZ domain containing family member 2, SEMCAP-2, PDZ domain-containing protein GIPC2, semaphorin cytoplasmic domain associated protein 2, semaF cytoplasmic domain associated protein 2, SEMCAP2, FLJ20075.
Appearance
Purity
Usage
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Description

Product Specs

Introduction
GIPC2, a 315 amino acid protein primarily located in the cytoplasm, contains a single PDZ domain. Highly expressed in the colon and kidney, with lower levels in adult liver, GIPC2 plays a crucial role in human gastric cancer development potentially through its involvement in growth factor signaling or cell adhesion processes.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For extended storage, store at -20°C. Repeated freeze-thaw cycles should be avoided.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The GIPC2 antibody has been validated for use in ELISA and Western blot applications, demonstrating high specificity and reactivity. Optimal working dilutions should be determined for each application and specific experimental conditions. For Western blot analysis, a starting dilution of 1:1000 is recommended.
Synonyms
GIPC PDZ domain containing family member 2, SEMCAP-2, PDZ domain-containing protein GIPC2, semaphorin cytoplasmic domain associated protein 2, semaF cytoplasmic domain associated protein 2, SEMCAP2, FLJ20075.
Purification Method
GIPC2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1G10AT.
Immunogen
Anti-human GIPC2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GIPC2 amino acids 1-315 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Gene and Protein Structure

The GIPC2 gene encodes a protein that consists of 315 amino acids . The protein has a central PDZ domain, which is a common structural domain in signaling proteins. This domain allows GIPC2 to interact with other proteins, facilitating various cellular functions. The GIPC2 protein shares a high degree of similarity with other GIPC family members, such as GIPC1, with which it shares 62.0% amino acid identity .

Function and Localization

GIPC2 is involved in several cellular processes, including signal transduction and protein trafficking. It is located in extracellular exosomes, which are small vesicles released by cells that play a role in cell communication . The protein’s ability to bind identical proteins suggests it may have a role in forming protein complexes that are essential for its function.

Research and Clinical Relevance

Research has shown that GIPC2 is associated with certain diseases, such as pheochromocytoma, a type of tumor that arises from the adrenal gland . The gene’s expression and function have been studied in various model organisms, including mice. Studies using mouse models have provided insights into the gene’s role in development and disease .

Antibody Development

The development of mouse anti-human GIPC2 antibodies has been crucial for research. These antibodies are used to study the protein’s expression and function in human tissues. They are also valuable tools for investigating the role of GIPC2 in diseases and for developing potential therapeutic strategies.

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