GHRL Human
Ghrelin Human Recombinant
Cat. No.
BT22281
Source
Escherichia Coli.
Synonyms
Appetite-regulating hormone precursor, Growth hormone secretagogue, Growth hormone-releasing peptide, GHRP, Motilin-related peptide, M46 protein, Ghrelin, Obestatin, MTLRP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Ghrelin Human Recombinant contains 115 amino acids (24-117 a.a.) and a total molecular mass of 12.8 kDa. The GHRL is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Obestatin, a hormone found in the stomach and small intestine lining of mammals, including humans, significantly suppresses appetite in mice. Similar effects are anticipated in humans. This peptide hormone, a relatively small protein, shares its encoding gene with ghrelin, a hunger-stimulating peptide hormone. The gene product splits into two smaller peptides: ghrelin and obestatin. Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, plays a role in regulating growth hormone release. Derived from a preprohormone called preproghrelin, which also generates obestatin, ghrelin acts as an endogenous ligand for the orphan G protein-coupled receptor GPR39, contributing to satiety and reduced food intake.
Description
Recombinant Human Ghrelin encompasses 115 amino acids (24-117 a.a.) with a total molecular mass of 12.8 kDa. An N-terminal 20 amino acid His Tag is fused to the GHRL, which is then purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The Ghrelin protein is supplied in a solution of 20mM Tris-HCl, pH 8.0, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Appetite-regulating hormone precursor, Growth hormone secretagogue, Growth hormone-releasing peptide, GHRP, Motilin-related peptide, M46 protein, Ghrelin, Obestatin, MTLRP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSFLSPEH QRVQQRKESK KPPAKLQPRA LAGWLRPEDG GQAEGAEDEM EVRFNAPFDV GIKLSGVQYQ QHSQALGKFL QDILWEEAKE APADK.
Product Science Overview
Introduction
Discovery and Nomenclature
Gene and Structure
The human ghrelin gene (GHRL) is located on chromosome 3 and consists of multiple exons . The gene produces a 117-amino acid precursor protein called preproghrelin, which is further processed to produce the active 28-amino acid ghrelin peptide . Ghrelin becomes active when octanoic acid is linked to serine at the 3-position by the enzyme ghrelin O-acyltransferase (GOAT) .
Functions
Ghrelin has several important functions:
- Appetite Regulation: Ghrelin levels increase before meals and decrease after eating, stimulating hunger and food intake .
- Growth Hormone Release: It stimulates the release of growth hormone from the anterior pituitary gland .
- Gastrointestinal Motility: Ghrelin enhances gastric motility and stimulates the secretion of gastric acid .
- Other Roles: Ghrelin is involved in regulating reward cognition, learning and memory, the sleep-wake cycle, taste sensation, and glucose metabolism .
Recombinant Ghrelin
Human recombinant ghrelin is a synthetic form of the naturally occurring hormone, produced using recombinant DNA technology. This involves inserting the ghrelin gene into bacterial or yeast cells, which then produce the hormone. Recombinant ghrelin is used in research to study its effects and potential therapeutic applications.
Therapeutic Potential
Ghrelin has shown promise in various therapeutic areas:
- Cachexia and Anorexia: Ghrelin’s appetite-stimulating properties make it a potential treatment for conditions like cachexia and anorexia .
- Growth Hormone Deficiency: It may be used to stimulate growth hormone release in individuals with deficiencies .
- Gastrointestinal Disorders: Ghrelin’s role in enhancing gastric motility suggests potential applications in treating gastrointestinal motility disorders .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
