GFRA3 Human, Sf9
GDNF Family Receptor Alpha3, GDNFR-alpha-3, GFR-alpha-3, GDNF Receptor Alpha-3, GDNFR3, GDNF Family Receptor Alpha-3, Glial Cell Line-Derived Neurotrophic Factor Receptor Alpha-3, GPI-Linked Receptor, GFRA3.
Greater than 85.0% as determined by SDS-PAGE.
Description
GFRA3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 585 amino acids (32-374) and having a molecular mass of 65.5kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). GFRA3 is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
GDNF Family Receptor Alpha3, GDNFR-alpha-3, GFR-alpha-3, GDNF Receptor Alpha-3, GDNFR3, GDNF Family Receptor Alpha-3, Glial Cell Line-Derived Neurotrophic Factor Receptor Alpha-3, GPI-Linked Receptor, GFRA3.
ADPDPLPTES RLMNSCLQAR RKCQADPTCS AAYHHLDSCT SSISTPLPSE EPSVPADCLE AAQQLRNSSL IGCMCHRRMK NQVACLDIYW TVHRARSLGN YELDVSPYED TVTSKPWKMN LSKLNMLKPD SDLCLKFAML CTLNDKCDRL RKAYGEACSG PHCQRHVCLR QLLTFFEKAA EPHAQGLLLC PCAPNDRGCG ERRRNTIAPN CALPPVAPNC LELRRLCFSD PLCRSRLVDF QTHCHPMDIL GTCATEQSRC LRAYLGLIGT AMTPNFVSNV NTSVALSCTC RGSGNLQEEC EMLEGFFSHN PCLTEAIAAK MRFHSQLFSQ DWPHPTFAVM AHQNENLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH.
Product Science Overview
GDNF Family Receptor Alpha 3 (GFRA3) is a member of the GDNF receptor family. It plays a crucial role in the signaling pathways that support the survival and development of neurons. GFRA3 forms a signaling receptor complex with the RET tyrosine kinase receptor and binds the ligand artemin (ARTN), a growth factor that supports the survival of sensory and sympathetic peripheral neurons .
The human recombinant GFRA3 protein is produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain containing 585 amino acids (32-374) and has a molecular mass of approximately 65.5 kDa. The protein is fused to a 239 amino acid hIgG-His-Tag at the C-terminus and is purified using proprietary chromatographic techniques .
GFRA3 is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor. It forms a signaling receptor complex with the RET tyrosine kinase receptor and binds the ligand artemin (ARTN). This binding leads to the autophosphorylation and activation of the RET receptor, which is essential for the survival and development of neurons .
The GFRA3 protein solution is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol. It should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and avoid multiple freeze-thaw cycles .
