GET4 Human

Golgi To ER Traffic Protein 4 Human Recombinant

Cat. No.

BT11629

Source

Escherichia Coli.

Synonyms

Golgi to ER traffic protein 4 homolog (S. cerevisiae), C7orf20, CEE, CGI-20, TRC35, Golgi to ER traffic protein 4 homolog, Conserved edge-expressed protein, Transmembrane domain recognition complex 35 kDa subunit, GET4.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

GET4 Human Recombinant produced in E. coli is. a single polypeptide chain containing 350 amino acids (1-327) and having a molecular mass of 38.9kDa. GET4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

The BAT3 complex is a group of proteins that help specific proteins, called tail-anchored (TA) proteins, reach their correct location within a cell. One of the proteins in the BAT3 complex is called GET4. TA proteins are special because they have a part that sits within the membrane of a cell compartment called the endoplasmic reticulum. The BAT3 complex helps these TA proteins get inserted into the endoplasmic reticulum membrane correctly. It works with another protein called ASNA1/TRC40 to make sure the TA proteins are delivered and inserted properly.

Description

This product contains the human version of the GET4 protein. It is made in a laboratory using E. coli bacteria. The protein is purified to a high degree and is provided in a solution. It is a single chain of 350 building blocks called amino acids, and it has a molecular weight of 38.9 kDa. For easier use and purification, it also contains a small tag of 23 additional amino acids at one end.

Physical Appearance

Clear solution that has been sterilized by filtration.

Formulation

The GET4 solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.

Stability

For short-term storage (up to 4 weeks), keep refrigerated at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing.

Purity

The purity is over 90% as determined by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLCLK NKSSASVVFT TYTQKHPSIE DGPPFVEPLL NFIWFLLLAV DGGKLTVFTV LCEQYQPSLR RDPMYNEYLD RIGQLFFGVP PKQTSSYGGL LGNLLTSLMG SSEQEDGEES PSDGSPIELD.

Product Science Overview

Function and Mechanism

GET4 is involved in the guided entry of tail-anchored proteins into the ER membrane. Tail-anchored proteins are characterized by a single C-terminal transmembrane region. The BAT3 complex, which includes GET4, helps maintain these proteins in a soluble state and ensures their proper delivery to the ER. This process is essential for the correct insertion of these proteins into the ER membrane.

The BAT3 complex interacts with newly synthesized tail-anchored proteins at the ribosome. Together with other proteins such as SGTA and ASNA1, GET4 mediates the delivery of these proteins to the ER. This complex also plays a role in the quality control of misfolded proteins, directing them either to the ER for proper folding or to the proteasome for degradation.

Biological Significance

GET4 is involved in several critical cellular processes, including:

Cytoplasmic sequestering of proteins: It helps in maintaining unfolded proteins involved in the ER-associated degradation (ERAD) pathway.
Maintenance of protein quality: By being part of the BAT3 complex, GET4 ensures that misfolded proteins are either correctly folded or degraded, thus maintaining cellular protein homeostasis.
Insertion of tail-anchored proteins: It facilitates the insertion of tail-anchored proteins into the ER membrane, which is vital for various cellular functions.

Clinical Relevance

Mutations or dysregulation of the GET4 gene can lead to several disorders. For instance, GET4 is associated with Congenital Disorder of Glycosylation, Type Iiy and Congenital Disorder of Glycosylation, Type In. These are genetic disorders that affect the glycosylation process, leading to a wide range of symptoms and developmental issues.

Research and Applications

Recombinant GET4 protein is used in various research applications to study its function and role in cellular processes. Understanding the mechanisms involving GET4 can provide insights into the development of therapeutic strategies for diseases related to protein misfolding and ER-associated degradation.

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GET4 Human

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