GDF10 Human
Growth differentiation factor 10 Human Recombinant
Cat. No.
BT17622
Source
Escherichia Coli.
Synonyms
Bone morphogenetic protein 3b, BMP-3b, Growth/differentiation factor 10, GDF-10, Bone-inducing protein, BIP, GDF10, BMP3B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GDF10 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids (369-478 a.a.) and having a total molecular mass of 12.5 kDa.
GDF10 is purified by proprietary chromatographic techniques.
GDF10 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Growth differentiation factor 10 (GDF10), a member of the bone morphogenetic protein (BMP) family within the transforming growth factor-beta (TGF-β) superfamily, plays a crucial role in skeletal development. Expressed in various tissues including the femur, brain, lung, skeletal muscle, pancreas, and testis, GDF10 contributes to head formation and potentially multiple aspects of skeletal morphogenesis. Human GDF10 mRNA is detected in the fetal cochlea and lung, as well as in adult tissues such as the testis, retina, pineal gland, and other neural tissues. GDF10, like other BMP family members, regulates cell growth and differentiation in both embryonic and adult tissues. These proteins share a characteristic polybasic proteolytic processing site, which upon cleavage, yields a mature protein containing seven conserved cysteine residues.
Description
Recombinant human GDF10, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 111 amino acids (residues 369-478). With a molecular mass of 12.5 kDa, it encompasses the bioactive region of GDF10. The protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The GDF10 solution is provided at a concentration of 1 mg/ml in a buffer containing 10 mM sodium citrate (pH 3.5), 1 mM dithiothreitol (DTT), 40% glycerol, and 0.1 M sodium chloride.
Stability
For short-term storage (up to 2-4 weeks), the GDF10 solution can be stored at 4°C. For extended storage, it is recommended to store the protein frozen at -20°C. To further enhance long-term stability, the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) is advised. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Purity
The purity of GDF10 is determined to be greater than 95% by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
Bone morphogenetic protein 3b, BMP-3b, Growth/differentiation factor 10, GDF-10, Bone-inducing protein, BIP, GDF10, BMP3B.
Source
Escherichia Coli.
Amino Acid Sequence
MQWDEPRVCS RRYLKVDFAD IGWNEWIISP KSFDAYYCAG ACEFPMPKIV RPSNHATIQS IVRAVGIIPG IPEPCCVPDK MNSLGVLFLD ENRNVVLKVY PNMSVDTCAC R.
Product Science Overview
Gene and Protein Structure
The GDF10 gene is located on chromosome 10 in humans . The protein encoded by this gene is a secreted ligand that binds to TGF-β receptors, leading to the recruitment and activation of SMAD family transcription factors . These transcription factors regulate gene expression and play crucial roles in various biological processes.
Biological Functions
GDF10 is closely related to Bone Morphogenetic Protein 3 (BMP3) and is involved in several key biological processes :
- Skeletal Morphogenesis: GDF10 plays a significant role in the development and formation of bones and skeletal structures .
- Head Formation: It is also implicated in the formation of head structures during embryonic development .
- Regulation of Apoptosis: GDF10 is involved in the regulation of programmed cell death, which is essential for maintaining cellular homeostasis .
- SMAD Protein Signal Transduction: It positively regulates the phosphorylation of SMAD proteins, which are critical mediators of TGF-β signaling pathways .
Expression Patterns
Recombinant Production
Recombinant GDF10 is produced using various expression systems, including bacterial and mammalian cells . The production of human recombinant growth factors, such as GDF10, involves the use of fusion partners to enhance the yield and solubility of the protein . These recombinant proteins are used in research and therapeutic applications due to their ability to regulate cellular processes such as growth, differentiation, and proliferation .
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