GCSF Monkey
Escherichia Coli.
CSF3, MGI-1G, GM-CSF beta, Pluripoietin, G-CSF, GCSF.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Granulocyte Colony Stimulating Rhesus Macaque Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18.9kDa.
GCSF is purified by proprietary chromatographic techniques.
Product Specs
Granulocyte colony-stimulating factor (GCSF) is a cytokine that regulates the production, differentiation, and function of granulocytes. It is found in the extracellular matrix and acts as a signaling molecule. There are three known transcript variants of the GCSF gene, which encode three distinct isoforms of the protein. GCSF belongs to the family of granulocyte/macrophage colony-stimulating factors (CSFs), which play a crucial role in hematopoiesis by regulating the production, differentiation, and function of granulocytes and monocytes-macrophages, two related white blood cell populations. Specifically, GCSF stimulates the production of granulocytes.
Recombinant Rhesus Macaque Granulocyte Colony Stimulating Factor, produced in E. coli, is a non-glycosylated polypeptide chain comprising 174 amino acids. With a molecular mass of approximately 18.9 kDa, it is purified using proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2µm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 7.4.
Reconstitute the lyophilized Granulocyte Colony Stimulating Rhesus Macaque in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Lyophilized GCSF, while stable at room temperature for 3 weeks, should be stored desiccated below -18°C. After reconstitution, store Granulocyte Colony Stimulating Rhesus Macaque at 4°C for 2-7 days. For long-term storage, keep it below -18°C. Avoid repeated freeze-thaw cycles.
Purity exceeds 98.0% as determined by:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
The half-maximal effective concentration (ED50), determined by a cell proliferation assay using murine NFS-60 cells, is less than 0.05 ng/ml. This corresponds to a specific activity greater than 2.0 × 10⁷ international units per milligram (IU/mg).
CSF3, MGI-1G, GM-CSF beta, Pluripoietin, G-CSF, GCSF.
Escherichia Coli.
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL RHSLGIPWAP LSSCPSQALQ LTGCLSQLHS SLFLYQGLLQ ALEGISPELS PTLDTLQLDI ADFATTIWQQ MEDLGMAPAL QPTQGAMPAF TSAFQRRAGG VLVASHLQRF LELAYRVLRH LAQS.
Product Science Overview
Granulocyte Colony Stimulating Factor (G-CSF) is a cytokine that plays a crucial role in hematopoiesis by controlling the production, differentiation, and function of granulocytes and monocytes-macrophages. The recombinant form of G-CSF derived from Rhesus Macaque (rRhG-CSF) is a significant tool in biomedical research due to its close genetic similarity to humans.
Recombinant Rhesus Macaque G-CSF (rRhG-CSF) is produced using Escherichia coli (E. coli) expression systems. It is a single non-glycosylated polypeptide chain containing 174 amino acids, with a molecular weight of approximately 18.9 kDa . The protein is highly purified, with a purity greater than 98% as determined by SDS-PAGE and HPLC analyses .
The biological activity of rRhG-CSF is measured by its ability to stimulate the proliferation of murine NFS-60 cells. The ED50 (effective dose for 50% of the population) is less than 0.05 ng/ml, corresponding to a specific activity of greater than 2.0 × 10^7 IU/mg . This high level of activity makes it a potent agent for stimulating granulocyte production.
The recombinant protein is typically lyophilized (freeze-dried) and reconstituted in sterile distilled water or an aqueous buffer containing 0.1% BSA (Bovine Serum Albumin) to a concentration of 0.1-1.0 mg/mL . It is recommended to store the reconstituted protein at -20°C to -70°C to maintain its stability and avoid repeated freeze-thaw cycles .
rRhG-CSF is widely used in research to study hematopoiesis, immune responses, and the effects of cytokines on various cell types. It is particularly valuable in preclinical studies involving non-human primates, as the Rhesus Macaque model closely mimics human physiology and immune responses.
