GCSAM Human

Germinal Center-Associated, Signaling and Motility Human Recombinant
Cat. No.
BT11341
Source
E.coli.
Synonyms
GCAT2, HGAL, Germinal center-associated signaling and motility protein, Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, GAL, GCET2 .
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GCSAM Human Recombinant produced in E. coli is a single polypeptide chain containing 201 amino acids (1-178) and having a molecular mass of 23 kDa. GCSAM is fused to 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GCSAM is a protein involved in signal transduction pathways, with elevated expression observed in germinal cell lymphomas. It possesses a potential PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites. Notably, interleukin-4 significantly induces GCSAM expression in B cells.
Description
Recombinant human GCSAM, produced in E. coli, is a single polypeptide chain comprising 201 amino acids (residues 1-178) with a molecular weight of 23 kDa. A 23 amino acid His-tag is fused to the N-terminus of GCSAM, and the protein is purified using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution.
Formulation
The GCSAM solution is supplied at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 85% via SDS-PAGE analysis.
Synonyms
GCAT2, HGAL, Germinal center-associated signaling and motility protein, Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, GAL, GCET2 .
Source
E.coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGNSLLR ENRRQQNTQE MPWNVRMQSP KQRTSRCWDH HIAEGCFCLP WKKILIFEKR QDSQNENERM SSTPIQDNVD QTYSEELCYT LINHRVLCTR PSGNSAEEYY ENVPCKAERP RESLGGTETE YSLLHMPSTD PRHARSPEDE YELLMPHRIS SHFLQQPRPL MAPSETQFSH L.

Product Science Overview

Introduction

Germinal Center-Associated, Signaling and Motility (GCSAM), also known as Human Germinal Center-Associated Lymphoma (HGAL), is a protein specifically expressed in germinal center lymphocytes. This protein plays a crucial role in regulating B-cell receptor signaling and cell motility, which are essential processes in the immune response and lymphomagenesis .

Structure and Function

GCSAM contains several important domains, including a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites . These domains suggest that GCSAM is involved in signal transduction pathways, which are critical for the regulation of cellular activities.

Role in Lymphoma

High expression of HGAL is associated with prolonged survival in patients with diffuse large B-cell lymphoma (DLBCL) and classical Hodgkin lymphoma (cHL) . HGAL inhibits lymphoma dissemination by interacting with multiple cytoskeletal proteins, thereby affecting cell motility through various mechanisms . Specifically, HGAL interacts with tubulin, which contributes to its effects on cell motility .

Pathophysiology

The cytoskeleton, functioning as a dynamic regulator of cellular shape and membrane organization in lymphocytes, plays a key role in B-cell motility and B-cell receptor (BCR) signaling . Tight regulation of the cytoskeleton is critical for intact and effective immunity. Alterations in cytoskeleton function may contribute to lymphoma pathogenesis . HGAL regulates B-cell receptor signaling and cell motility by interacting with cytoskeletal proteins, thereby influencing lymphoma motility and spread .

Clinical Significance

HGAL is a reliable marker for diagnosing diffuse large B-cell lymphoma, particularly in cases where CD10 is negative . It is also useful in diagnosing T follicular helper (TFH) cell-derived lymphomas, including angioimmunoblastic T-cell lymphoma . The expression of HGAL is associated with early-stage DLBCL, potentially limiting lymphoma dissemination and improving patient survival .

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