GCLM Antibody
Description
Product Specs
Product Science Overview
Glutamate-Cysteine Ligase (GCL) is a critical enzyme in the biosynthesis of glutathione, a major antioxidant in cellular defense mechanisms. The enzyme is composed of two subunits: the catalytic subunit (GCLC) and the modifier subunit (GCLM). The modifier subunit, GCLM, enhances the catalytic efficiency of GCLC, forming a functional holoenzyme complex. This article delves into the background of the GCLM, particularly focusing on the mouse anti-human variant.
GCLM is a protein-coding gene that plays a pivotal role in glutathione synthesis. The enzyme’s activity is crucial for maintaining cellular redox balance and protecting cells from oxidative stress. GCLM, in conjunction with GCLC, catalyzes the first and rate-limiting step in glutathione biosynthesis, which involves the ligation of glutamate and cysteine to form γ-glutamylcysteine .
The GCLM subunit is essential for the optimal functioning of the GCL enzyme. It enhances the catalytic activity of GCLC, thereby increasing the overall efficiency of glutathione production. Glutathione is vital for detoxifying reactive oxygen species (ROS) and maintaining cellular homeostasis. Deficiencies in GCLM can lead to reduced glutathione levels, resulting in increased susceptibility to oxidative stress and related pathologies .
Mouse anti-human GCLM antibodies are monoclonal antibodies developed to specifically target the human GCLM protein. These antibodies are used in various research applications, including Western blotting, immunoprecipitation, and immunohistochemistry. They are valuable tools for studying the expression and function of GCLM in different biological contexts .
Research involving GCLM has significant implications for understanding oxidative stress-related diseases. Studies using GCLM knockout mice have shown that the absence of this subunit leads to decreased glutathione levels and increased oxidative damage in tissues. This model has been instrumental in elucidating the role of GCLM in various physiological and pathological processes .
