GAP43 Human

Growth Associated Protein 43 Human Recombinant
Cat. No.
BT11014
Source
E.coli.
Synonyms
Growth associated protein 43, Axonal membrane protein GAP-43, Neural phosphoprotein B-50, calmodulin-binding protein P-57, nerve growth-related peptide GAP43, neuromodulin, protein F1, pp46, B-50.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GAP43 Human Recombinant produced in E. coli is a single polypeptide chain containing 262 amino acids (1-238) and having a molecular mass of 27.3kDa (Molecular size on SDS-PAGE will appear higher).
GAP43 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GAP43 is a phosphoprotein found in the presynaptic membrane that plays a role in synaptic reorganization and neural development. It is highly concentrated in neuronal growth cones during development and axonal regeneration. GAP43 is also present in specific areas of the mature nervous system and is crucial for maintaining long-term potentiation (LTP).
Description
Recombinant human GAP43, expressed in E. coli, is a single polypeptide chain consisting of 262 amino acids (residues 1-238). It has a molecular weight of 27.3 kDa. Note that the protein's size on SDS-PAGE may appear larger due to glycosylation. GAP43 is fused to a 24 amino acid His-tag at its N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The GAP43 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Growth associated protein 43, Axonal membrane protein GAP-43, Neural phosphoprotein B-50, calmodulin-binding protein P-57, nerve growth-related peptide GAP43, neuromodulin, protein F1, pp46, B-50.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLCCMR RTKQVEKNDD DQKIEQDGIK PEDKAHKAAT KIQASFRGHI TRKKLKGEKK DDVQAAEAEA NKKDEAPVAD GVEKKGEGTT TAEAAPATGS KPDEPGKAGE TPSEEKKGEG DAATEQAAPQ APASSEEKAG SAETESATKA STDNSPSSKA EDAPAKEEPK QADVPAAVTA AAATTPAAED AAAKATAQPP TETGESSQAE ENIEAVDETK PKESARQDEG KEEEPEADQE HA

Product Science Overview

Structure and Function

GAP-43 is a nervous tissue-specific cytoplasmic protein that can attach to the membrane via a dual palmitoylation sequence on cysteines 3 and 4 . It is a major component of the motile “growth cones” that form the tips of elongating axons . These growth cones are essential for the differentiation of neurons and play a significant role in axonal and dendritic filopodia induction .

The protein is involved in several critical biological processes, including:

  • Neurite outgrowth: Essential for the differentiation of neurons .
  • Synaptic plasticity: GAP-43 is a marker of synaptic plasticity and is critical for normal development of the serotonergic innervation .
  • Axonal regeneration: Its synthesis is upregulated during axonal regeneration .
Recombinant Human GAP-43

Recombinant human GAP-43 is produced using DNA sequences encoding the human GAP43 protein. This recombinant protein is often tagged with a polyhistidine tag at the C-terminus to facilitate purification . The recombinant human GAP-43 consists of 249 amino acids and has a predicted molecular mass of 26.2 kDa. Due to glycosylation, the apparent molecular mass is approximately 47 kDa in SDS-PAGE under reducing conditions .

Applications

Recombinant human GAP-43 is used in various research applications, including:

  • Neuroscience research: Studying neuronal growth, differentiation, and regeneration.
  • Drug development: Investigating potential therapeutic targets for neurodegenerative diseases.
  • Developmental biology: Understanding the role of GAP-43 in brain development and synaptic plasticity.
Storage and Stability

Recombinant human GAP-43 is typically provided as a lyophilized powder and should be stored under sterile conditions at -20°C to -80°C. It is recommended to aliquot the protein for optimal storage and avoid repeated freeze-thaw cycles .

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