GALE Antibody
Description
Product Specs
Product Science Overview
Mouse Anti Human Antibodies are secondary antibodies that are generated by immunizing mice with human immunoglobulins. These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins .
Function and Applications
Mouse Anti Human Antibodies are commonly used in various laboratory techniques, including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry . These secondary antibodies offer increased versatility, enabling users to employ many detection systems such as horseradish peroxidase (HRP), alkaline phosphatase (AP), and fluorescence . They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody .
Production and Purification
The production of Mouse Anti Human Antibodies involves immunizing the host animal (mouse) with a pooled population of human immunoglobulins. These antibodies can be further purified and modified through techniques such as immunoaffinity chromatography, antibody fragmentation, and label conjugation to generate highly specific reagents .
Clinical Relevance
In some cases, the use of mouse antibodies in humans can lead to the development of Human Anti-Mouse Antibodies (HAMA). The HAMA response is essentially an allergic reaction to the mouse antibodies, which can range from mild forms like a rash to more severe and life-threatening responses such as kidney failure .
