The GAGA-POZ protein, also known as the GAGA factor, is a sequence-specific DNA-binding protein found in Drosophila melanogaster (fruit fly). It plays a crucial role in the regulation of various genes, including developmentally regulated genes, stress-induced genes, cell cycle-regulated genes, and housekeeping genes .
The GAGA factor contains two main domains:
The recombinant GAGA-POZ protein is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 130 amino acids with a molecular mass of 14 kDa . The protein is typically formulated in a sterile, colorless solution containing 10mM HEPES (pH 7.4) and 25mM NaCl .
GAGA-POZ is used in various laboratory research applications, including:
For short-term storage (2-4 weeks), the protein should be kept at 4°C. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability .