GADD45GIP1 Human

Growth Arrest and DNA-Damage-Inducible Gamma Interacting Protein 1 Human Recombinant
Cat. No.
BT30790
Source
E.coli.
Synonyms
Growth arrest and DNA-damage-inducible gamma interacting protein 1, PRG6, CRIF1, PLINP-1, KBBP2, Plinp1, Papillomavirus L2-interacting nuclear protein 1, CKII beta-associating protein, CR6-interacting factor 1, p53-responsive gene 6 protein, CKII beta binding protein 2, growth arrest and DNA damage-inducible proteins-interacting protein 1, papillomavirus L2 interacting nuclear protein 1, PLINP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GADD45GIP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (48-222) and having a molecular mass of 22.6 kDa.
GADD45GIP1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GADD45GIP1, found in the nucleus, plays a role in regulating apoptosis. It is present in various tissues like the heart, thyroid, trachea, kidney, ovary, pancreas, testis, and stomach. This protein acts as a negative regulator of the G1 to S phase transition in the cell cycle. It achieves this by working with GADD45 proteins to suppress the activity of cyclin-dependent kinases.
Description
Recombinant human GADD45GIP1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 196 amino acids (residues 48-222) and has a molecular weight of 22.6 kDa. This protein is fused to a 21 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The provided GADD45GIP1 solution has a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 2mM DTT, 0.2M NaCl, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be kept at 4°C. For longer storage, it should be frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined by SDS-PAGE analysis and is greater than 90%.
Synonyms
Growth arrest and DNA-damage-inducible gamma interacting protein 1, PRG6, CRIF1, PLINP-1, KBBP2, Plinp1, Papillomavirus L2-interacting nuclear protein 1, CKII beta-associating protein, CR6-interacting factor 1, p53-responsive gene 6 protein, CKII beta binding protein 2, growth arrest and DNA damage-inducible proteins-interacting protein 1, papillomavirus L2 interacting nuclear protein 1, PLINP1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPRWQLGPRY AAKQFARYGA ASGVVPGSLW PSPEQLRELE AEEREWYPSL ATMQESLRVK QLAEEQKRRE REQHIAECMA KMPQMIVNWQ QQQRENWEKA QADKERRARL QAEAQELLGY QVDPRSARFQ ELLQDLEKKE RKRLKEEKQK RKKEARAAAL AAAVAQDPAA SGAPSS

Product Science Overview

Introduction

Growth Arrest and DNA-Damage-Inducible Gamma Interacting Protein 1 (GADD45GIP1) is a protein that plays a crucial role in the cellular response to stress, particularly in the context of DNA damage and growth arrest. This protein is encoded by the GADD45GIP1 gene in humans and is involved in various cellular processes, including cell cycle regulation, apoptosis, and DNA repair.

Gene and Protein Structure

The GADD45GIP1 gene is located on chromosome 9 in humans. The protein encoded by this gene is known for its interaction with the GADD45 family of proteins, which are involved in the cellular response to DNA damage and stress. The GADD45GIP1 protein itself is composed of 222 amino acids and has a molecular weight of approximately 25 kDa .

Function and Mechanism

GADD45GIP1 is primarily involved in the regulation of the cell cycle and apoptosis. It mediates the activation of the p38/JNK pathway via the MTK1/MEKK4 kinase, which is crucial for the cellular response to environmental stresses . This protein is highly expressed in the placenta and plays a significant role in the regulation of growth and apoptosis.

Biological Processes

GADD45GIP1 is involved in several key biological processes, including:

  • Cell Cycle Regulation: It helps in controlling the progression of the cell cycle, particularly in response to DNA damage.
  • Apoptosis: GADD45GIP1 is involved in the induction of programmed cell death, which is essential for eliminating damaged or cancerous cells.
  • DNA Repair: It plays a role in the repair of damaged DNA, ensuring the maintenance of genomic stability .
Clinical Significance

The expression of GADD45GIP1 has been linked to various diseases and conditions. For instance, low levels of GADD45GIP1 expression have been associated with several types of cancer, indicating its potential role as a tumor suppressor . Additionally, alterations in the expression of this protein have been observed in conditions such as pituitary adenoma and chromium allergic contact dermatitis .

Research and Applications

Recombinant human GADD45GIP1 is used in various research applications to study its role in cellular stress responses, DNA repair mechanisms, and its potential as a therapeutic target in cancer treatment. The availability of recombinant proteins allows researchers to conduct in-depth studies on the function and regulation of GADD45GIP1 in different cellular contexts.

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THE BIOTEK
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