G6PD Antibody
Description
Product Specs
Product Science Overview
Glucose-6-Phosphate Dehydrogenase (G6PD) is a crucial enzyme in the pentose phosphate pathway, which is essential for cellular metabolism. This enzyme catalyzes the conversion of glucose-6-phosphate into 6-phosphoglucono-δ-lactone while reducing NADP+ to NADPH. The NADPH produced is vital for protecting cells against oxidative damage by maintaining the level of reduced glutathione .
G6PD is a cytosolic enzyme that plays a significant role in the pentose phosphate pathway, which provides reducing energy to cells by maintaining the level of NADPH. NADPH, in turn, helps in maintaining the level of reduced glutathione, which protects red blood cells from oxidative damage . The enzyme is also involved in the Entner-Doudoroff pathway, a type of glycolysis .
G6PD deficiency is an X-linked genetic disorder that affects millions of people worldwide. This deficiency makes individuals prone to non-immune hemolytic anemia, especially under oxidative stress conditions such as infections, certain medications, or ingestion of fava beans . The deficiency is particularly prevalent in regions where malaria is or was common, as the condition provides some protection against malaria .
Mouse anti-human G6PD antibodies are used in various research and diagnostic applications. These antibodies are typically produced by immunizing mice with human G6PD protein, leading to the generation of monoclonal or polyclonal antibodies that specifically recognize human G6PD . These antibodies can be used in techniques such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA) to detect and quantify G6PD in human samples .
Humanized mouse models of G6PD deficiency have been developed to study the effects of this condition in vivo. These models involve the introduction of human G6PD genes into mice, allowing researchers to study the physiological and biochemical impacts of G6PD deficiency under controlled conditions . Such models are invaluable for testing the hemolytic potential of drugs and understanding the molecular mechanisms underlying G6PD deficiency .
