G3BP1 Human

GTPase Activating Protein (SH3 domain) Binding Protein 1 Human Recombinant
Cat. No.
BT24197
Source
Escherichia Coli.
Synonyms
Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP, HDH-VIII, MGC111040.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

G3BP1 Human Recombinant fused with an 8 amino acid His tag at C-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 474 amino acids (1-466 a.a.) and having a molecular mass of 53.2kDa.
The G3BP1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
G3BP1, a member of the heterogeneous nuclear RNA-binding protein family, plays a crucial role in the Ras signal transduction pathway. This DNA-unwinding enzyme exhibits a preference for partially unwound 3'-tailed substrates and possesses the ability to unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent manner. G3BP1 specifically interacts with the Ras-GTPase-activating protein through its SH3 domain. Furthermore, G3BP1 exhibits selective cleavage activity, exclusively cleaving between cytosine and adenine, with a preference for MYC mRNA at the 3'-UTR.
Description
Recombinant human G3BP1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 474 amino acids (1-466 a.a.), including an 8 amino acid His tag fused at the C-terminus, and has a molecular weight of 53.2 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The G3BP1 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, 2mM DTT, and 100mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the G3BP1 protein is determined to be greater than 85.0% based on SDS-PAGE analysis.
Synonyms
Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP, HDH-VIII, MGC111040.
Source
Escherichia Coli.
Amino Acid Sequence
MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY VHGGLDSNGK PADAVYGQKE IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN NNQALRRFMQ TFVLAPEGSV ANKFYVHNDI FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQKS SSPAPADIAQ TVQEDLRTFS WASVTSKNLP PSGAVPVTGI PPHVVKVPAS QPRPESKPES QIPPQRPQRD QRVREQRINI PPQRGPRPIR EAGEQGDIEP RRMVRHPDSH QLFIGNLPHE VDKSELKDFF QSYGNVVELR INSGGKLPNF GFVVFDDSEP VQKVLSNRPI MFRGEVRLNV EEKKTRAARE GDRRDNRLRG PGGPRGGLGG GMRGPPRGGM VQKPGFGVGR GLAPRQVEHH HHHH.

Product Science Overview

Introduction

GTPase Activating Protein (SH3 domain) Binding Protein 1, commonly referred to as G3BP1, is a multifunctional protein that plays a crucial role in various cellular processes. It is encoded by the G3BP1 gene located on chromosome 5q33.1 in humans . G3BP1 is known for its involvement in stress granule assembly, RNA binding, and regulation of microRNA processing.

Structure and Function

G3BP1 is a member of the heterogeneous nuclear RNA-binding proteins and is involved in the Ras signal transduction pathway . It has several key domains, including the SH3 domain, which allows it to interact with other proteins and regulate their activity. G3BP1 can bind to partially unwound DNA and RNA substrates, and it exhibits endonuclease activity .

One of the primary functions of G3BP1 is its role in stress granule formation. Stress granules are cytoplasmic aggregates of proteins and RNAs that form in response to cellular stress. G3BP1 is a key component of these granules and helps in their assembly by interacting with other proteins .

Role in MicroRNA Processing

G3BP1 has been shown to regulate the processing of microRNA-1 (miR-1) during cardiac hypertrophy . During cardiac hypertrophy, G3BP1 is upregulated and binds to the consensus sequence in the pre-miR-1-2 stem-loop, restricting the processing of miR-1. This results in a decrease in mature miR-1 levels and an increase in the expression of its target genes, which are essential for transcription and translation .

Interaction with Other Proteins

G3BP1 interacts with various proteins to regulate different cellular processes. For example, it interacts with USP10, a deubiquitinating enzyme, and SND1, a component of the RNA-induced silencing complex . These interactions are crucial for the regulation of stress granule assembly and other cellular functions.

Clinical Significance

The dysregulation of G3BP1 has been implicated in several diseases, including cancer and cardiac hypertrophy. Its role in stress granule formation and microRNA processing makes it a potential target for therapeutic interventions. Understanding the function and regulation of G3BP1 can provide insights into the development of novel treatments for these conditions.

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