FUCA2 Human

The recombinant form of this enzyme is produced using Chinese Hamster Ovary (CHO) cells. The human plasma alpha-L-fucosidase/FUCA2 protein is expressed with an N-terminal 6-His tag, which facilitates its purification . The recombinant protein has a predicted molecular mass of approximately 52 kDa, and it is typically supplied as a 0.2 μm filtered solution in Tris and NaCl .

Fucosidase Alpha-L-2 Plasma is essential for the hydrolysis of fucose residues from glycoproteins and glycolipids. This activity is critical for various biological processes, including cell-cell interaction, signal transduction, and immune response . The enzyme’s activity is measured by its ability to cleave a fluorogenic substrate, 4-methylumbelliferyl-alpha-L-fucopyranoside, with a specific activity of over 550 pmol/min/μg .

The enzyme represents 10-20% of the total cellular fucosidase activity in human plasma . It is particularly important for the adhesion of Helicobacter pylori to human gastric cancer cells, highlighting its role in pathogenic interactions . Additionally, mutations in the FUCA2 gene have been associated with developmental and epileptic encephalopathies .

The recombinant enzyme is stable for six months from the date of receipt when stored at -20 to -70°C. It is important to avoid repeated freeze-thaw cycles to maintain its activity .