FTSJ2 Human

FtsJ RNA Methyltransferase Homolog 2 Human Recombinant
Cat. No.
BT10428
Source
E.coli.
Synonyms
FtsJ RNA methyltransferase homolog 2 (E. coli), putative ribosomal RNA methyltransferase 2, MRM2 RNA methyltransferase homolog, rRNA (uridine-2'-O-)-methyltransferase, cell division protein FtsJ, Protein ftsJ homolog 2, MRM2, FJH1, EC 2.1.1.-.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FTSJ2 Human Recombinant produced in E. coli is a single polypeptide chain containing 219 amino acids (51-246) and having a molecular mass of 24.1 kDa.
FTSJ2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
FTSJ2 (FtsJ RNA Methyltransferase Homolog 2) is a member of the S-adenosylmethionine-binding protein family. This nucleolar protein plays a role in cell cycle regulation, DNA repair, and the processing and modification of rRNA. FTSJ2 is widely expressed, with the highest levels found in muscle, placenta, and heart tissues.
Description
Recombinant human FTSJ2, expressed in E. coli, is a single polypeptide chain consisting of 219 amino acids (residues 51-246). It has a molecular weight of 24.1 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The FTSJ2 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 1mM DTT, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the FTSJ2 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
FtsJ RNA methyltransferase homolog 2 (E. coli), putative ribosomal RNA methyltransferase 2, MRM2 RNA methyltransferase homolog, rRNA (uridine-2'-O-)-methyltransferase, cell division protein FtsJ, Protein ftsJ homolog 2, MRM2, FJH1, EC 2.1.1.-.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSYRCRSA FKLLEVNERH QILRPGLRVL DCGAAPGAWS QVAVQKVNAA GTDPSSPVGF VLGVDLLHIF PLEGATFLCP ADVTDPRTSQ RILEVLPGRR ADVILSDMAP NATGFRDLDH DRLISLCLTL LSVTPDILQP GGTFLCKTWA GSQSRRLQRR LTEEFQNVRI IKPEASRKES SEVYFLATQY HGRKGTVKQ

Product Science Overview

Structure and Expression

FTSJ2 is a nucleolar protein that plays a crucial role in the processing and modification of ribosomal RNA (rRNA). It is involved in the formation of 2’-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA . The protein is extensively expressed in various tissues, with the highest expression levels observed in the muscle, placenta, and heart .

Functional Role

FTSJ2 is implicated in several cellular processes, including:

  • Cell Cycle Control: It is involved in the regulation of the cell cycle, ensuring proper cell division and proliferation .
  • DNA Repair: FTSJ2 plays a role in the DNA repair mechanisms, maintaining genomic stability and integrity .
  • rRNA Processing: The protein is essential for the processing and modification of rRNA, which is critical for the proper functioning of ribosomes and protein synthesis .
Recombinant FTSJ2

Recombinant human FTSJ2 is produced using Escherichia coli expression systems. The recombinant protein typically includes an N-terminal His-tag for purification purposes and is used in various research applications, including SDS-PAGE and mass spectrometry (MS) . The recombinant form of FTSJ2 is highly purified, with a purity level exceeding 95% .

Applications in Research

Recombinant FTSJ2 is widely used in scientific research to study its function and role in cellular processes. It is also utilized in the development of assays and experiments aimed at understanding the mechanisms of rRNA modification and the impact of these modifications on cellular physiology .

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