FGFR1OP N-terminal like (FOPNL) is a protein that belongs to the FGFR1OP family. It is known for its role in the biogenesis of cilia, which are hair-like structures on the surface of cells that play crucial roles in cell movement and signaling . FOPNL is extensively expressed in various tissues, including the brain, heart, kidney, liver, lung, skeletal muscle, placenta, and intestine .
The FGFR1OP gene encodes a largely hydrophilic protein that is postulated to be a member of the leucine-rich protein family . The gene is located on chromosome 6 in humans and chromosome 17 in mice . The protein contains one LisH domain, which is involved in protein-protein interactions and is essential for the protein’s function in cilia biogenesis .
FGFR1OP N-terminal like protein plays a significant role in normal cell proliferation and differentiation, particularly in the erythroid lineage . It is involved in various cellular processes, including the regulation of protein kinase activity, cell migration, cell growth, and the organization of cell projections . The protein is also crucial for the G2/M transition of the mitotic cell cycle and the anchoring of microtubules .
A chromosomal translocation involving the FGFR1OP gene and the fibroblast growth factor receptor 1 (FGFR1) gene has been identified in cases of myeloproliferative disorder . This translocation results in a chimeric protein that contains the N-terminal leucine-rich region of FGFR1OP fused to the catalytic domain of FGFR1 . This chimeric protein is thought to contribute to the abnormal proliferation and differentiation observed in myeloproliferative disorders .
FGFR1OP N-terminal like (Human Recombinant) is produced in Escherichia coli (E. coli) as a single polypeptide chain containing 194 amino acids, with a molecular mass of 21.9 kDa . The recombinant protein is fused to a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques . The protein is available in various quantities and is used primarily for laboratory research purposes .