FOLH1 Mouse

Folate Hydrolase 1 Mouse Recombinant
Cat. No.
BT27878
Source
Sf9, Baculovirus cells.
Synonyms
Folh1, GCP2, mopsm, Glutamate carboxypeptidase 2, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Naalad1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FOLH1 Mouse Recombinant produced in in Sf9 Baculovirus cells is a single, non-glycosylated polypeptide chain containing 717 amino acids (45-752a.a) and having a molecular mass of 80.5kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). FOLH1 is fused to a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Folate Hydrolase 1 (Folh1) is a single-pass type 2 membrane protein primarily found in prostate epithelium. As a member of the peptidase M28 family and M28B subfamily, Folh1 exhibits both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase activity. Its presence extends to the urinary bladder, kidney, testis, ovary, stomach, small intestine, colon, and the capillary endothelium of various tumors. Due to its distribution, Folh1 has potential applications in targeted imaging and treatment of recurrent or metastatic disease.
Description
Recombinant Mouse FOLH1, produced in Sf9 Baculovirus cells, is a single, non-glycosylated polypeptide chain comprising 717 amino acids (45-752a.a). It has a molecular mass of 80.5 kDa and exhibits a migration pattern of 70-100 kDa on SDS-PAGE under reducing conditions. The protein includes a C-terminal 6-amino acid His-tag and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The FOLH1 solution is provided at a concentration of 0.5 mg/ml and contains Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
Folh1, GCP2, mopsm, Glutamate carboxypeptidase 2, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Naalad1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPKPSNEAT GNVSHSGMKK EFLHELKAEN IKKFLYNFTR TPHLAGTQNN FELAKQIHDQ WKEFGLDLVE LSHYDVLLSY PNKTHPNYIS IINEDGNEIF KTSLSEQPPP GYENISDVVP PYSAFSPQGT PEGDLVYVNY ARTEDFFKLE REMKISCSGK IVIARYGKVF RGNMVKNAQL AGAKGMILYS DPADYFVPAV KSYPDGWNLP GGGVQRGNVL NLNGAGDPLT PGYPANEHAY RHELTNAVGL PSIPVHPIGY DDAQKLLEHM GGPAPPDSSW KGGLKVPYNV GPGFAGNFST QKVKMHIHSY TKVTRIYNVI GTLKGALEPD RYVILGGHRD AWVFGGIDPQ SGAAVVHEIV RSFGTLKKKG RRPRRTILFA SWDAEEFGLL GSTEWAEEHS RLLQERGVAY INADSSIEGN YTLRVDCTPL MYSLVYNLTK ELQSPDEGFE GKSLYDSWKE KSPSPEFIGM PRISKLGSGN DFEVFFQRLG IASGRARYTK NWKTNKVSSY PLYHSVYETY ELVVKFYDPT FKYHLTVAQV RGAMVFELAN SIVLPFDCQS YAVALKKYAD TIYNISMKHP QEMKAYMISF DSLFSAVNNF TDVASKFNQR LQELDKSNPI LLRIMNDQLM YLERAFIDPL GLPGRPFYRH IIYAPSSHNK YAGESFPGIY DALFDISSKV NASKAWNEVK RQISIATFTV QAAAETLREV AHHHHHH.

Product Science Overview

Structure and Function

FOLH1 is a multifunctional enzyme that primarily acts as a glutamate carboxypeptidase. It hydrolyzes poly-γ-glutamated folates to release folate, which can then be taken up by cells via various folate transporters . This enzymatic activity is crucial for folate metabolism, which is essential for DNA synthesis and repair, as well as for cellular division and growth.

In the context of cancer, particularly prostate cancer, FOLH1 plays a significant role. The enzyme is overexpressed in prostate cancer cells and is involved in the metabolism of folates, which may contribute to cancer progression . This overexpression makes FOLH1 a valuable target for imaging and therapeutic strategies in prostate cancer treatment.

Recombinant Mouse FOLH1

Recombinant mouse FOLH1 is produced using Chinese Hamster Ovary (CHO) cell lines. The recombinant protein is typically tagged with a 6-His tag at the N-terminal for purification purposes. The molecular mass of the recombinant protein is approximately 80 kDa, but it appears as a 100 kDa band under reducing conditions in SDS-PAGE due to glycosylation .

The activity of recombinant mouse FOLH1 is measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. This activity is quantified using fluorescence after derivatization with ortho-phthaldialdehyde .

Applications

Recombinant mouse FOLH1 is used in various research applications, including:

  • Cancer Research: Due to its overexpression in prostate cancer, FOLH1 is used as a target for developing imaging agents and therapeutic strategies.
  • Folate Metabolism Studies: The enzyme’s role in folate metabolism makes it a valuable tool for studying cellular folate uptake and utilization.
  • Drug Development: FOLH1 inhibitors are being explored as potential therapeutic agents for treating prostate cancer and other conditions involving folate metabolism.

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