FOLH1 Human
Sf9, Baculovirus cells.
Glutamate carboxypeptidase 2 isoform 1,Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpolygamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Nacetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma glutamate carboxypeptidase, Folh1, FGCP, FOLH, GCP2, GCPII, mGCP, NAALAD1, NAALAdase, PSM, PSMA
Greater than 90.0% as determined by SDS-PAGE.
Description
FOLH1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 717 amino acids (44-750 a.a) and having a molecular mass of 80.7kDa.
FOLH1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
FOLH1, also called glutamate carboxypeptidase 2 (GCPII), is a protein found in the membrane of cells. It belongs to the peptidase M28 family. It is highly concentrated in the prostate, but is also found in other organs like the ovaries, liver, stomach, intestines, bladder, kidneys, testes, and in the blood vessels of some tumors. This makes it potentially useful for imaging and treating cancer that has spread. FOLH1 acts like a pair of scissors, breaking down a molecule called N-acetylaspartylglutamate into glutamate and N-acetylaspartate. This process requires zinc.
This version of FOLH1 is produced in insect cells. It is a single chain of 717 building blocks called amino acids, with a molecular weight of 80.7kDa. It includes a 6 amino acid His tag for purification purposes. The protein has been purified using specialized techniques.
This solution contains 0.25mg/ml of FOLH1 protein in a special salt solution (Phosphate buffered saline, pH 7.4) with 20% glycerol.
For short-term storage (up to 4 weeks), keep at refrigerated at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freezing and thawing.
More than 90% pure as measured by SDS-PAGE.
Glutamate carboxypeptidase 2 isoform 1,Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpolygamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Nacetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma glutamate carboxypeptidase, Folh1, FGCP, FOLH, GCP2, GCPII, mGCP, NAALAD1, NAALAdase, PSM, PSMA
Sf9, Baculovirus cells.
ADPMKSSNEA TNITPKHNMK AFLDELKAEN IKKFLYNFTQ IPHLAGTEQN FQLAKQIQSQ WKEFGLDSVE LAHYDVLLSY PNKTHPNYIS IINEDGNEIF NTSLFEPPPP GYENVSDIVP PFSAFSPQGM PEGDLVYVNY ARTEDFFKLE RDMKINCSGK IVIARYGKVF RGNKVKNAQL AGAKGVILYS DPADYFAPGV KSYPDGWNLP GGGVQRGNIL NLNGAGDPLT PGYPANEYAY RRGIAEAVGL PSIPVHPIGY YDAQKLLEKM GGSAPPDSSW RGSLKVPYNV GPGFTGNFST QKVKMHIHST NEVTRIYNVI GTLRGAVEPD RYVILGGHRD SWVFGGIDPQ SGAAVVHEIV RSFGTLKKEG WRPRRTILFA SWDAEEFGLL GSTEWAEENS RLLQERGVAY INADSSIEGN YTLRVDCTPL MYSLVHNLTK ELKSPDEGFE GKSLYESWTK KSPSPEFSGM PRISKLGSGN DFEVFFQRLG IASGRARYTK NWETNKFSGY PLYHSVYETY ELVEKFYDPM FKYHLTVAQV RGGMVFELAN SIVLPFDCRD YAVVLRKYAD KIYSISMKHP QEMKTYSVSF DSLFSAVKNF TEIASKFSER LQDFDKSNPI VLRMMNDQLM FLERAFIDPL GLPDRPFYRH VIYAPSSHNK YAGESFPGIY DALFDIESKV DPSKAWGEVK RQIYVAAFTV QAAAETLSEV AHHHHHH
Product Science Overview
Folate Hydrolase 1 (FOLH1), also known as Prostate-Specific Membrane Antigen (PSMA), is a type II transmembrane zinc metallopeptidase. It is encoded by the FOLH1 gene and is primarily expressed in the nervous system, prostate, kidney, and small intestine . FOLH1 plays a crucial role in the metabolism of folates, which are essential for DNA synthesis and repair, as well as cell division and growth .
FOLH1 is highly expressed in the prostate, and its expression increases with the grade of prostate cancer and in metastatic disease . This makes it a valuable biomarker for prostate cancer diagnosis and a target for imaging and therapeutic strategies . Additionally, FOLH1 is involved in the hydrolysis of poly-γ-glutamated folates to folate, which can then be taken up by cells via various folate transporters .
Recombinant human FOLH1 is produced using DNA sequences encoding the human FOLH1 protein. These sequences are expressed in host cells, such as HEK293 cells, to produce the recombinant protein . The recombinant protein is then purified to achieve high purity levels, typically greater than 95%, as determined by SDS-PAGE . The recombinant FOLH1 protein is often tagged with a polyhistidine tag to facilitate purification and detection .
The industrial production of recombinant FOLH1 involves several key steps:
- Gene Cloning: The gene encoding FOLH1 is cloned into an expression vector.
- Transformation: The expression vector is introduced into host cells, such as HEK293 cells.
- Protein Expression: The host cells are cultured under conditions that promote the expression of the recombinant FOLH1 protein.
- Protein Purification: The recombinant protein is purified using techniques such as affinity chromatography, which exploits the polyhistidine tag for efficient purification .
- Quality Control: The purity and activity of the recombinant protein are assessed using various analytical methods, including SDS-PAGE and activity assays .
FOLH1 functions as a glutamate carboxypeptidase, hydrolyzing substrates such as N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu . This enzymatic activity is crucial for the metabolism of folates and the regulation of folate levels within cells . The activity of recombinant FOLH1 can be measured using fluorescence-based assays, where the product L-Glu is derivatized and detected .
