FNDC5 Human, Yeast
Description
FNDC5 is purified by proprietary chromatographic techniques.
Product Specs
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__N__K__D__E__V__T__M__K__E.Product Science Overview
FNDC5 is a protein-coding gene that encodes a type I transmembrane glycoprotein. The protein contains a fibronectin type III domain, which is a common structural motif found in a variety of proteins involved in cell adhesion, growth, and differentiation . The FNDC5 gene is located on chromosome 1 in humans .
One of the most notable aspects of FNDC5 is its cleaved form, known as irisin. Irisin is released into the bloodstream during physical exercise and has been implicated in the conversion of white adipose tissue to brown adipose tissue, a process known as "browning" . This conversion is significant because brown fat burns energy and generates heat, which can help in weight management and metabolic health.
FNDC5 is expressed in various tissues, but it is most prominently found in muscle tissue. During exercise, the expression of FNDC5 increases, leading to higher levels of irisin in the bloodstream . Irisin has been shown to have several beneficial effects, including improving glucose homeostasis and lipid metabolism .
The recombinant form of FNDC5, produced in yeast, is a glycosylated polypeptide chain containing 110 amino acids and has a molecular mass of 20-25 kDa . This recombinant protein is often used in research to study its biological activity and potential therapeutic applications. The yeast expression system is advantageous because it allows for the production of large quantities of the protein with proper post-translational modifications .
The recombinant FNDC5 protein is typically lyophilized (freeze-dried) for stability and can be stored at room temperature for up to three weeks. For long-term storage, it is recommended to keep the protein desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C for short-term use and below -18°C for long-term use .
Given its role in metabolic processes, FNDC5/irisin has been studied for its potential therapeutic applications in treating metabolic disorders such as obesity and type 2 diabetes. Additionally, its ability to induce the browning of white adipose tissue makes it a promising candidate for weight management therapies .
