Greater than 95.0% as determined by analysis by SDS-PAGE.
The product is lyophilized from a sterile aqueous solution with a pH of 0.1% TFA and filtered through a 0.2-micron filter.
To reconstitute the lyophilized FGF-8, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions.
Lyophilized FGF-8 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. Once reconstituted, FGF-8 should be stored at 4°C for 2-7 days or below -18°C for extended periods. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. It is crucial to avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of FGF-8 is greater than 95.0% as determined by SDS-PAGE analysis.
The ED50, determined by the product's ability to induce proliferation in NR6-R 3T3 cells, is 0.915 ng/ml. This corresponds to a specific activity of 1.1 x 106 units/mg.
In humans, FGF-8 exists in four isoforms: FGF-8a, FGF-8b, FGF-8e, and FGF-8f . These isoforms are generated through alternative splicing of the FGF-8 mRNA. Among these, FGF-8b is the predominant form and has been shown to possess significant oncogenic transforming capacity . The amino acid sequence of human FGF-8b shares 100% identity with its mouse counterpart .
FGF-8 plays a pivotal role in embryogenesis, influencing the development of the brain, limbs, heart, and facial structures during gastrulation . It is also involved in the differentiation of embryonic stem cells into neural progenitor cells and further into dopaminergic neurons . Additionally, FGF-8 stimulates the proliferation of various cell types, including osteoblasts, endothelial cells, and myogenic cells .
FGF-8 is known to support the androgen and anchorage-independent growth of mammary tumor cells . Overexpression of FGF-8 has been associated with increased tumor growth and angiogenesis . This protein’s expression, once thought to be restricted to the testes and ovaries in adults, has been observed in several other organ systems .