FGF17 Mouse

Fibroblast Growth Factor 17 Mouse Recombinant
Cat. No.
BT17265
Source

Escherichia Coli.

Synonyms

Fibroblast growth factor 17, FGF-17, FGF17, FGF-13, HH20.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Fibroblast Growth Factor 17 Mouse Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 194 amino acid and having a molecular mass of approximately 22.5kDa.
FGF17 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Fibroblast Growth Factor 17 (FGF17) belongs to the fibroblast growth factor family. Members of the FGF family possess broad mitogenic and cell survival activities, playing crucial roles in various biological processes such as morphogenesis, embryonic development, cell growth, tissue repair, and tumor growth and invasion. The FGF17 gene exhibits high expression levels in the cerebellum and cortex. Notably, the mouse homolog of the FGF17 gene is localized to specific regions within the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton, and developing arteries. This suggests its involvement in the development of the central nervous system, bone, and vascular systems.
Description
Recombinant Fibroblast Growth Factor 17 from Mouse, produced in E. coli, is a non-glycosylated polypeptide chain composed of 194 amino acids. With a molecular mass of approximately 22.5 kDa, FGF17 undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 0.02% Tween-20 and 700 mM NaCl.
Solubility
To reconstitute the lyophilized Fibroblast Growth Factor 17, it is recommended to dissolve it in sterile PBS at a concentration of at least 100µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized FGF17, while stable at room temperature for up to 3 weeks, should be stored desiccated at a temperature below -18°C. Once reconstituted, Fibroblast Growth Factor 17 should be stored at 4°C for a period of 2-7 days. For long-term storage, it is recommended to store it at -18°C. It is important to avoid repeated freeze-thaw cycles.
Purity
Greater than 98.0% as determined by: Analysis using RP-HPLC and SDS-PAGE.
Biological Activity
The ED50, as determined by a cell proliferation assay employing murine balb/c 3T3 cells, is less than 10 ng/ml. This corresponds to a specific activity greater than 1.0 × 10^5 IU/mg in the presence of 10 µg/ml of heparin.
Synonyms

Fibroblast growth factor 17, FGF-17, FGF17, FGF-13, HH20.

Source

Escherichia Coli.

Amino Acid Sequence

TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT.

Product Science Overview

Structure and Biochemical Properties

FGF-17 is a medium-sized single-chain protein with a molecular weight of approximately 22.5 kDa . It possesses a conserved core of about 120 amino acids, which is a characteristic feature of the FGF family . The recombinant form of FGF-17, produced in E. coli, is a non-glycosylated polypeptide chain containing 194 amino acids .

Functions and Mechanisms

FGF-17 functions by binding to and activating high-affinity FGF receptors (FGFR) on target cells. This binding initiates a signal transduction cascade involving kinases and transcription factors . The activation of these signaling pathways results in various cellular effects, such as:

  • Mitogenesis: The stimulation of cell division.
  • Differentiation: The process by which cells become specialized.
  • Migration: The movement of cells to different locations.
  • Angiogenesis: The formation of new blood vessels.
  • Wound Healing: The repair of damaged tissues .
Applications in Biomedical Research

Recombinant FGF-17 is widely used in biomedical research due to its ability to maintain, expand, and differentiate diverse types of cells in culture . Some specific applications include:

  • Maintenance of Pluripotent Stem Cells: FGF-17 helps in maintaining the undifferentiated state of stem cells.
  • Differentiation of Stem Cells: It aids in the differentiation of stem cells into specific cell types, such as insulin-producing pancreatic-like cells.
  • Expansion of Neural Stem and Progenitor Cells: FGF-17 is used to expand neural stem cells and progenitor cells .
Production and Purification

The recombinant form of FGF-17 is produced in E. coli and purified using proprietary chromatographic techniques . The protein is typically lyophilized from a filtered solution and can be reconstituted in phosphate-buffered saline (PBS) for use in various applications .

Stability and Storage

Recombinant FGF-17 is stable for 12 months when stored at -20 to -70°C as supplied. After reconstitution, it should be stored under sterile conditions at 2 to 8°C for up to one month or at -20 to -70°C for up to three months .

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