FGF16 Human
Fibroblast Growth Factor 16 Human Recombinant
Cat. No.
BT8528
Source
Escherichia Coli.
Synonyms
Fibroblast Growth Factor 16, Metacarpal 4-5 Fusion, FGF-16, MF4, FGF16.
Appearance
Sterile Filtered colorless clear solution.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Description
FGF16 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a molecular mass of 23.6kDa.
The FGF-16 is purified by proprietary chromatographic techniques.
The FGF-16 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Fibroblast growth factor 16 (FGF16) belongs to the extensive family of fibroblast growth factors. These factors share the characteristic of binding to heparin and possess a core FGF domain comprising 120 amino acids, contributing to their conserved tertiary structure. In humans, FGF16 is initially translated as a precursor protein of 207 amino acids, containing a single site for N-linked glycosylation. Despite lacking a typical signal peptide, FGF16 demonstrates efficient production through alternative mechanisms that deviate from the conventional protein secretion pathway. Notably, FGF16 expression is observed in cardiac cells, highlighting its crucial role in the intricate process of heart development. Furthermore, mutations in the FGF16 gene have been linked to individuals exhibiting metacarpal 4-5 fusion. FGF16 emerges as a pivotal regulator of embryonic development, exerting control over cell proliferation and differentiation. Its significance is underscored by its indispensable role in ensuring normal cardiomyocyte proliferation and overall heart development.
Description
Recombinant human FGF16, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 206 amino acids, with a molecular weight of 23.6 kDa. This FGF-16 variant is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
FGF-16 is supplied as a 0.2 μm filtered solution in 20mM Tris-HCl buffer (pH 9.0) containing 1M NaCl, 0.2% Tween-20, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein (either HSA or BSA) to a final concentration of 0.1% is advisable. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of this product is greater than 98.0%, as determined by two analytical methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) and (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The ED50, determined by a thymidine uptake assay using BaF3 cells transfected with FGF receptors, is less than 0.5 ng/mL. This corresponds to a specific activity greater than 2.0 x 10^6 IU/mg.
Synonyms
Fibroblast Growth Factor 16, Metacarpal 4-5 Fusion, FGF-16, MF4, FGF16.
Source
Escherichia Coli.
Amino Acid Sequence
AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR.
Product Science Overview
Structure and Characteristics
FGF-16 is a heparin-binding growth factor. The human recombinant form of FGF-16 is produced in Escherichia coli (E. coli) and consists of 206 amino acids, resulting in a molecular mass of approximately 23.6 kDa . The protein is typically purified using proprietary chromatographic techniques to ensure high purity and activity .
Biological Functions
FGF-16 plays a crucial role in several physiological processes:
- Prenatal Development: FGF-16 is essential during prenatal development, particularly in the development of the central nervous system .
- Postnatal Growth and Regeneration: It promotes cellular proliferation and differentiation, which are vital for tissue growth and regeneration .
- Cardioprotection: FGF-16 has been shown to play a critical role in embryonic heart development and is thought to have cardioprotective effects after birth .
- Adipocyte Proliferation: In vitro studies have demonstrated that FGF-16 promotes the proliferation of brown adipocytes .
Mechanism of Action
Applications in Research
Human recombinant FGF-16 is widely used in research to study its effects on various cell types and to understand its role in different biological processes. It is particularly valuable in studies related to:
- Stem Cell Biology: FGF-16 is used to investigate its role in stem cell differentiation and proliferation .
- Cardiovascular Research: Researchers study FGF-16 to explore its cardioprotective properties and its potential therapeutic applications in heart diseases .
- Adipocyte Biology: FGF-16 is used to understand its effects on adipocyte proliferation and its potential role in metabolic regulation .
Storage and Handling
The lyophilized form of human recombinant FGF-16 should be stored at -20°C for long-term storage. Upon reconstitution, it is stable for up to one week at 2°C to 8°C. For maximal stability, it is recommended to apportion the reconstituted preparation into working aliquots and store them at -20°C, avoiding repeated freeze/thaw cycles .
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