FGF14 Human

Fibroblast Growth Factor 14 Human Recombinant

Cat. No.

BT8441

Source

E.coli.

Synonyms

Fibroblast growth factor 14, FGF-14, Fibroblast growth factor homologous factor 4, FHF-4, FGF14, FHF4, SCA27, bA397O8.2.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

FGF14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 271 amino acids (1-247 a.a) and having a molecular mass of 30kDa.
FGF14 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Fibroblast Growth Factor 14 (FGF14) is a member of the fibroblast growth factor (FGF) family. FGFs are known for their roles in mitogenesis and cell survival, and they participate in a wide array of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, and tumor progression including growth and invasion. Mutations in the FGF14 gene have been associated with autosomal dominant cerebral ataxia.

Description

Recombinant Human FGF14, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses 271 amino acids, including amino acids 1-247 of the FGF14 sequence, and has a molecular weight of 30 kDa. A 24 amino acid His-tag is fused to the N-terminus of the FGF14 protein. Purification is achieved through proprietary chromatographic techniques.

Physical Appearance

A clear, colorless solution, sterile-filtered.

Formulation

The FGF14 protein solution is provided at a concentration of 0.25 mg/ml. The solution is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, and 5 mM DTT.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain product integrity.

Purity

Purity is determined to be greater than 90% using SDS-PAGE analysis.

Synonyms

Fibroblast growth factor 14, FGF-14, Fibroblast growth factor homologous factor 4, FHF-4, FGF14, FHF4, SCA27, bA397O8.2.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAAAIA SGLIRQKRQA REQHWDRPSA SRRRSSPSKN RGLCNGNLVD IFSKVRIFGL KKRRLRRQDP QLKGIVTRLY CRQGYYLQMH PDGALDGTKD DSTNSTLFNL IPVGLRVVAI QGVKTGLYIA MNGEGYLYPS ELFTPECKFK ESVFENYYVI YSSMLYRQQE SGRAWFLGLN KEGQAMKGNR VKKTKPAAHF LPKPLEVAMY REPSLHDVGE TVPKPGVTPS KSTSASAIMN GGKPVNKSKT T.

Product Science Overview

Introduction

Fibroblast Growth Factor 14 (FGF14) is a member of the fibroblast growth factor family, which is known for its broad mitogenic and cell survival activities. These growth factors are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF14, in particular, plays a crucial role in the central nervous system.

Structure and Function

FGF14 is a protein that is encoded by the FGF14 gene in humans. It is part of the intracellular FGF subfamily, which also includes FGF11, FGF12, and FGF13. Unlike other FGFs that function as secreted signaling molecules, FGF14 operates within cells. It interacts with voltage-gated sodium channels and is essential for the proper functioning of neurons.

The primary structure of FGF14 consists of a single polypeptide chain. The recombinant form of FGF14 is produced using recombinant DNA technology, where the FGF14 gene is inserted into a suitable expression system, such as E. coli or mammalian cells, to produce the protein in large quantities.

Biological Role

FGF14 is predominantly expressed in the brain and spinal cord. It is involved in the regulation of neuronal excitability and synaptic transmission. FGF14 binds to the intracellular C-terminal domain of voltage-gated sodium channels, influencing their activity and, consequently, the electrical signaling in neurons.

Mutations in the FGF14 gene have been linked to neurological disorders, such as spinocerebellar ataxia type 27 (SCA27), which is characterized by progressive problems with movement and coordination. This highlights the importance of FGF14 in maintaining normal neurological function.

Applications in Research and Medicine

Recombinant FGF14 is used extensively in research to study its role in neuronal function and its potential implications in neurological diseases. By understanding how FGF14 interacts with sodium channels and other cellular components, researchers can develop targeted therapies for conditions like SCA27 and other neurodegenerative diseases.

In addition, FGF14 has potential therapeutic applications. For instance, modulating FGF14 activity could be a strategy to treat certain neurological disorders. However, more research is needed to fully understand its therapeutic potential and to develop safe and effective treatments.

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FGF14 Human

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