FETUB Human

Fetuin-B Human Recombinant

Cat. No.

BT9190

Source

HEK 293.

Synonyms

Fetuin-B, 16G2, Fetuin-like protein IRL685, Gugu, FETUB, IRL685.

Appearance

Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Fetuin-B Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain containing a total of 377 amino acids, having a molecular mass of 41.8kDa (calculated) though it migrates at approximately 55kDa on SDS PAGE, the Fetuin-B is also composed of a 4 a.a N-terminal linker and fused to a 6 a.a His tag at C-Terminus.
The Human FETUB is purified by proprietary chromatographic techniques.

Product Specs

Introduction

Fetuin-B (FETUB) is a protein that belongs to the fetuin family, which is part of the cystatin superfamily. It contains two cystatin domains and is secreted by cells, primarily in the liver, tongue, and placenta. FETUB plays a crucial role in regulating bone formation by inhibiting mineralization. Fetuins, including FETUB, are involved in various biological processes such as bone formation and breakdown, regulating hepatocyte growth factor receptors, and responding to inflammation. Moreover, FETUB is vital for oocyte maturation, particularly in preventing the zona pellucida (ZP) from hardening prematurely before fertilization. It achieves this by inhibiting the activity of the protease ASTL, which is responsible for cleaving ZP2 and triggering ZP hardening.

Description

Recombinant Human Fetuin-B is produced in HEK cells. It is a single, glycosylated polypeptide chain with a molecular weight of 41.8 kDa (calculated) and comprises 377 amino acids. It includes a 4-amino acid N-terminal linker and a 6-amino acid His tag at the C-terminus. On SDS-PAGE, it migrates at approximately 55 kDa. The purification process involves proprietary chromatographic techniques to ensure high purity.

Physical Appearance

White, lyophilized powder after filtration.

Formulation

The Fetuin-B protein undergoes filtration (0.4 μm) and is subsequently lyophilized from a solution containing 0.5 mg/ml of Fetuin-B in a buffer of 0.05 M phosphate and 0.075 M NaCl, at a pH of 7.4.

Solubility

To prepare a working solution, add deionized water to the lyophilized powder to a final concentration of 0.5 mg/ml. Allow the pellet to dissolve completely. Please be aware that this product is not sterile. Before using it in cell culture, filter the solution using an appropriate sterile filter.

Stability

Store the lyophilized protein at -20°C. After reconstitution, aliquot the protein and store it at 4°C to minimize freeze-thaw cycles. The reconstituted protein remains stable for a limited period at 4°C, showing no significant change for up to two weeks.

Purity

The purity of the protein is greater than 90.0%, as determined by SDS-PAGE analysis.

Synonyms

Fetuin-B, 16G2, Fetuin-like protein IRL685, Gugu, FETUB, IRL685.

Source

HEK 293.

Amino Acid Sequence

HSHVCGAMSP PQLALNPSAL LSRGCNDSDV LAVAGFALRD INKDRKDGYV LRLNRVNDAQ EYRRGGLGSL FYLTLDVLET DCHVLRKKAW QDCGMRIFFE SVYGQCKAIF YMNNPSRVLY LAAYNCTLRP VSKKKIYMTC PDCPSSIPTD SSNHQVLEAA TESLAKYNNE NTSKQYSLFK VTRASSQWVV GPSYFVEYLI KESPCTKSQA SSCSLQSSDS VPVGLCKGSL TRTHWEKFVS VTCDFFESQA PATGSENSAV NQKPTNLPKV EESQQKNTPP TDSPSKAGPR GSVQYLPDLD DKNSQEKGPQ EAFPVHLDLT TNPQGETLDI SFLFLEPMEE KLVVLPFPKE KARTAECPGP AQNASPLVLP PHHHHHH.

Product Science Overview

Structure and Function

Fetuin-B’s structure includes two cystatin domains (CY1 and CY2) connected by a linker, with a CPDCP-trunk and a C-terminal region (CTR). The protein is involved in several biological processes, including female fertility, metabolism, and liver disease. It acts as a protease inhibitor, which is crucial for egg fertilization and preventing premature zona pellucida hardening before fertilization. This is likely achieved by inhibiting the protease activity of ASTL, a protease that mediates the cleavage.

Expression and Production

Recombinant human Fetuin-B is typically produced in HEK293 cells. The DNA sequence encoding the human FETUB (NP_055190.2) (Met 1-Pro 382) is expressed with a polyhistidine tag at the C-terminus. The recombinant human FETUB consists of 375 amino acids and has a predicted molecular mass of 41.7 kDa. Due to glycosylation, it migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.

Applications and Stability

Recombinant human Fetuin-B is used in various research applications, including studies on fertility, metabolism, and liver disease. It is typically supplied as a lyophilized powder and is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein for optimal storage and avoid repeated freeze-thaw cycles.

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FETUB Human

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