FeLV
Description
The E.Coli truncated derived recombinant protein contains the p27 immunodominant regions, containing 248 amino acids (271-519) and having a Mw of 31kda as visualized by SDS-PAGE. Recombinant Feline Leukemia Virus p27 is fused to a His Tag and purified by conventional chromatography techniques.
Product Specs
Product Science Overview
Feline Leukemia Virus (FeLV) is a retrovirus that infects cats, leading to various health issues, including immunosuppression, anemia, and lymphoma. One of the key components of FeLV is the p27 protein, which plays a crucial role in the virus’s structure and replication. The recombinant form of this protein, known as FeLV p27 recombinant, is widely used in research and diagnostic applications.
The p27 protein is a major structural component of FeLV, encoded by the gag gene. It is integral to the virus’s ability to replicate and assemble new viral particles. The protein is highly immunogenic, meaning it elicits a strong immune response in infected cats. This characteristic makes p27 a valuable target for diagnostic tests, such as enzyme-linked immunosorbent assays (ELISAs) and immunochromatographic tests, which detect the presence of this antigen in blood samples to confirm FeLV infection .
Recombinant p27 is produced using genetic engineering techniques. The gene encoding the p27 protein is inserted into a suitable expression system, such as Escherichia coli (E. coli), which then produces the protein. The recombinant p27 protein is often fused with a his-tag at its N-terminal to facilitate purification. The resulting protein is then purified and tested for quality and purity .
Recombinant p27 is used in various applications, including:
- Diagnostic Tests: As mentioned earlier, p27 is a key target for diagnostic tests to detect FeLV infection in cats. These tests are crucial for early detection and management of the disease.
- Research: Recombinant p27 is used in research to study the structure and function of the protein, as well as its interactions with other viral and host proteins. This research provides insights into the pathogenesis of FeLV and the development of FeLV-related diseases .
- Vaccine Development: Understanding the immunogenic properties of p27 can aid in the development of vaccines against FeLV. Recombinant p27 can be used as an antigen in vaccine formulations to stimulate an immune response in cats .
Recombinant p27 is subjected to rigorous quality control measures to ensure its purity and functionality. The protein is typically analyzed using SDS-PAGE electrophoresis to confirm its purity, which is usually greater than 95% . The protein is stored in a liquid solution or as a lyophilized powder, and it is recommended to aliquot and store it at -20°C to -80°C to avoid repeated freezing and thawing cycles .
