FCER1A Human, HEK
HEK293.
FCERIA, FCERA, Fc epsilon receptor Ia, Fcepsilon RI-alpha, Fc epsilon RI alpha chain, FcERI, High affinity immunoglobulin epsilon receptor subunit alpha isoform1, IgE Fc receptor subunit alpha, FCER1A, FCE1A.
Greater than 90% as determined by SDS-PAGE.
Description
FCER1A Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 186 amino acids (26-205aa) and having a molecular mass of 21.8kDa. FCER1A is fused to a 6 His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Fc-Epsilon RI-Alpha (FcεRIα) is a crucial component of the high-affinity IgE receptor, which plays a significant role in allergic reactions and immune response. The recombinant form of this protein, produced in Human Embryonic Kidney (HEK) cells, is widely used in research to study its structure, function, and potential therapeutic applications.
FcεRIα is a type I transmembrane glycoprotein belonging to the multichain immune recognition receptor (MIRR) family . It is the alpha subunit of the high-affinity IgE receptor, which also includes beta and gamma subunits. The primary function of FcεRIα is to bind immunoglobulin E (IgE) with high affinity, facilitating the immune response to allergens .
Upon binding to IgE, FcεRIα initiates a cascade of signaling pathways that lead to the activation and differentiation of myeloid cells, such as mast cells and basophils . This activation results in the release of histamines and other inflammatory mediators, contributing to allergic reactions and asthma .
The recombinant form of FcεRIα is produced in HEK cells to ensure proper folding, post-translational modifications, and bioactivity. HEK cells are commonly used for recombinant protein production due to their ability to perform complex glycosylation and other modifications that are essential for the protein’s function .
Recombinant FcεRIα produced in HEK cells is typically purified to high levels of purity (>95%) and tested for bioactivity using functional assays such as ELISA . This ensures that the recombinant protein retains its ability to bind IgE and initiate signaling pathways effectively.
Recombinant FcεRIα is used in various research applications, including:
- Allergy Research: Studying the mechanisms of allergic reactions and identifying potential therapeutic targets.
- Drug Development: Screening for compounds that can inhibit the interaction between FcεRIα and IgE, potentially leading to new treatments for allergies and asthma.
- Structural Studies: Investigating the structure of FcεRIα to understand its binding interactions with IgE and other molecules.
