FBP2 Antibody
Description
Product Specs
Product Science Overview
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human antigens. These antibodies are used in various research and diagnostic applications to detect, sort, or purify human proteins .
Production and Applications
Mouse anti-human antibodies are produced by immunizing mice with human immunoglobulins or other human proteins. The antibodies are then harvested and purified using techniques such as immunoaffinity chromatography . These secondary antibodies can be conjugated with various labels, such as enzymes (HRP, AP), fluorophores (FITC, PE), or biotin, to facilitate detection in different assays .
Uses in Research and Diagnostics
These antibodies are widely used in techniques such as Western blotting, ELISA, flow cytometry, and immunohistochemistry . They offer increased sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody . This amplification is crucial for detecting low-abundance proteins in complex samples.
Human Anti-Mouse Antibody (HAMA) Response
One potential issue with using mouse anti-human antibodies is the development of the Human Anti-Mouse Antibody (HAMA) response. This response occurs when the human immune system recognizes the mouse antibodies as foreign and mounts an immune reaction against them . The HAMA response can range from mild allergic reactions to severe complications, such as kidney failure .
