FASLG Human

FAS Ligand Human Recombinant
Cat. No.
BT22536
Source
Escherichia Coli.
Synonyms
Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178, FASLG, APT1LG1, CD95L, TNFSF6, ALPS1B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FASLG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (130-281 a.a.) and having a molecular mass of 19.6kDa.
FASLG is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
FASLG, a type II transmembrane protein, belongs to the tumor necrosis factor (TNF) superfamily and plays a crucial role in immune system regulation and cancer development. This protein, expressed on activated T cells as a non-disulfide-linked homotrimer, initiates apoptosis in Fas-expressing cells upon binding to Fas/CD95/TNFRSF6. Metalloproteinases facilitate the release of FASLG from the cell surface as a soluble, trimeric molecule. While retaining its Fas-binding ability, the soluble form exhibits significantly reduced apoptotic activity. Furthermore, FASLG interacts with DcR3, a soluble decoy receptor lacking signal transduction capabilities. Impaired Fas-mediated apoptosis contributes to oncogenesis and drug resistance in existing tumors. The constitutive expression of FASLG in various tumors allows them to evade immune surveillance. Notably, both mouse and human FASLG demonstrate activity on cells from both species.
Description
Recombinant human FASLG, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 173 amino acids (130-281 a.a.). With a molecular weight of 19.6 kDa, it features a 21 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The FASLG protein solution (1 mg/ml) is supplied in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178, FASLG, APT1LG1, CD95L, TNFSF6, ALPS1B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MQIGHPSPPP EKKELRKVAH LTGKSNSRSM PLEWEDTYGI VLLSGVKYKK GGLVINETGL YFVYSKVYFR GQSCNNLPLS HKVYMRNSKY PQDLVMMEGK MMSYCTTGQM WARSSYLGAV FNLTSADHLY VNVSELSLVN FEESQTFFGL YKL.

Product Science Overview

Structure and Function

FasL is a homotrimeric protein, meaning it consists of three identical polypeptide chains . It has several key structural features:

  • Cytoplasmic Domain: This region is involved in intracellular signaling.
  • Stalk Region: Connects the cytoplasmic domain to the transmembrane domain.
  • Transmembrane Domain ™: Anchors the protein to the cell membrane.
  • TNF Homology Domain (THD): Responsible for the homotrimerization of FasL.
  • C-terminal Region: Involved in binding to the Fas receptor (CD95) .

FasL exists in both membrane-bound and soluble forms . The membrane-bound form is primarily responsible for inducing apoptosis by binding to its receptor, Fas (CD95), on target cells . This interaction leads to the formation of the death-inducing signaling complex (DISC), which ultimately results in cell death .

Role in the Immune System

FasL is expressed on various cell types, including:

  • Cytotoxic T Lymphocytes
  • Natural Killer (NK) Cells
  • Monocytes
  • Neutrophils
  • Vascular Endothelial Cells

The primary function of FasL is to maintain immune system homeostasis by inducing apoptosis in target cells. This process is essential for eliminating infected or cancerous cells and for regulating immune responses .

Recombinant Fas Ligand

Recombinant human Fas Ligand is produced using various expression systems, such as Chinese Hamster Ovary (CHO) cells or HEK293 cells . The recombinant protein is often used in research to study apoptosis and immune regulation. It is also utilized in cytotoxicity assays to evaluate the apoptotic potential of various compounds .

Recombinant Fas Ligand is typically purified to high levels of purity and is available in both carrier-free and carrier-containing formulations . The carrier protein, often Bovine Serum Albumin (BSA), enhances protein stability and shelf-life .

Applications in Research

Recombinant Fas Ligand is widely used in scientific research to:

  • Study Apoptosis: Understanding the mechanisms of programmed cell death.
  • Investigate Immune Responses: Exploring how FasL regulates immune cell interactions.
  • Develop Therapeutics: Potential applications in cancer therapy and autoimmune diseases .

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