FABP5 Human, His

FABP5 is a single, non-glycosylated polypeptide chain containing 135 amino acids and has a molecular mass of approximately 19.66 kDa . The human recombinant version of FABP5 is produced in Escherichia coli (E. coli) and is fused to a His tag at the N-terminus, which facilitates its purification using standard chromatography techniques .

FABP5 plays a crucial role in intracellular transport of long-chain fatty acids and related active lipids. It selectively delivers specific fatty acids from the cytosol to the nucleus, where they activate nuclear receptors such as peroxisome proliferator-activated receptor delta (PPARδ). This activation promotes cell proliferation and survival . Additionally, FABP5 is involved in the transport of retinoic acid to nuclear receptors, modulating inflammation by regulating prostaglandin E2 (PGE2) biosynthesis during inflammatory responses .

FABP5 was first identified as being upregulated in psoriasis tissue, indicating its potential role in skin-related disorders . Its involvement in keratinocyte differentiation and inflammation suggests that it may be a target for therapeutic interventions in skin diseases and inflammatory conditions .

The human recombinant FABP5 with His tag is widely used in research to study its function and role in various biological processes. It is utilized in applications such as SDS-PAGE and other biochemical assays to investigate its interactions with fatty acids and other ligands .

The recombinant FABP5 protein is supplied in a sterile filtered colorless liquid formulation containing 20mM Tris HCl (pH 8) and 50% glycerol. It should be stored at -20°C for long-term storage and at 4°C if used within 2-4 weeks. It is important to avoid freeze-thaw cycles to maintain its stability .