FABP2 Human, His

Fatty Acid Binding Protein 2 (FABP2), also known as Intestinal-type Fatty Acid Binding Protein (I-FABP), is a member of the fatty acid-binding protein family. These proteins are involved in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is specifically expressed in the small intestine and plays a crucial role in the absorption and metabolism of dietary fatty acids.

The recombinant human FABP2 protein is typically produced in Escherichia coli (E. coli) expression systems. It is a single, non-glycosylated polypeptide chain consisting of 132 amino acids and has a molecular mass of approximately 17.3 kDa . The recombinant version often includes a His tag at the N-terminus, which facilitates purification through affinity chromatography techniques .

FABP2 binds saturated long-chain fatty acids with high affinity, while it binds unsaturated long-chain fatty acids with lower affinity . This protein is believed to be involved in the synthesis of triglyceride-rich lipoproteins and may also function as a lipid sensor, helping to maintain energy homeostasis . By transporting fatty acids within cells, FABP2 plays a critical role in lipid metabolism and energy balance.

Recombinant human FABP2 with a His tag is widely used in various research applications, including:

• sELISA (sandwich ELISA): It serves as a standard protein for quantifying FABP2 levels in biological samples .
• SDS-PAGE: It is used as a molecular weight marker and for studying protein-protein interactions .
• Western Blot: It acts as a positive control in immunological assays .