EYA2 Human

Eyes Absent Homolog 2 Human Recombinant
Cat. No.
BT7796
Source
E.coli.
Synonyms
Eyes absent homolog 2 (Drosophila), EAB1, EC 3.1.3.48.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EYA2 Human Recombinant produced in E. coli is a single polypeptide chain containing 295 amino acids (244-514) and having a molecular mass of 33.2kDa.
EYA2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
EYA2, a member of the tyrosine phosphatase family, plays a crucial role in DNA repair. It specifically dephosphorylates histone H2AX at tyrosine 142 (H2AXY142ph), facilitating the recruitment of DNA repair complexes containing MDC1. This dephosphorylation activity is essential for efficient DNA repair. Additionally, EYA2's histone phosphatase function contributes to its role in regulating transcription during organogenesis.
Description
Recombinant EYA2, produced in E. coli, is a single polypeptide chain with a molecular weight of 33.2 kDa. This protein consists of 295 amino acids (residues 244-514) and includes a 24 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The EYA2 solution is provided at a concentration of 0.5 mg/ml and contains the following components: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of EYA2 is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
Eyes absent homolog 2 (Drosophila), EAB1, EC 3.1.3.48.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL.

Product Science Overview

Gene and Protein Structure

The EYA2 gene is located on chromosome 20 (20q13.12) in humans . The gene produces multiple transcript variants encoding different isoforms of the EYA2 protein . The full-length human recombinant EYA2 protein consists of 538 amino acids and is often tagged with GST (Glutathione S-transferase) at the N-terminal for research purposes .

Function and Mechanism

EYA2 functions as a transcriptional coactivator and a phosphatase. It has been shown to play a crucial role in eye development and other developmental processes. In particular, EYA2 is involved in the regulation of transcription, DNA repair, and cellular response to DNA damage .

One of the key functions of EYA2 is its role as a tyrosine phosphatase. It specifically dephosphorylates ‘Tyr-142’ of histone H2AX (H2AXY142ph), which is essential for efficient DNA repair. By dephosphorylating H2AX, EYA2 promotes the recruitment of DNA repair complexes containing MDC1, thereby facilitating the DNA repair process .

Biological Processes

EYA2 is involved in several biological processes, including:

  • Striated muscle tissue development
  • Regulation of transcription (DNA-templated)
  • Protein dephosphorylation
  • Cellular response to DNA damage stimulus
  • Multicellular organism development
  • Extrinsic apoptotic signaling pathway in the absence of ligand
  • Mesodermal cell fate specification
  • Mitochondrial outer membrane permeabilization
  • Positive regulation of DNA repair
  • Peptidyl-tyrosine dephosphorylation
  • Chromatin organization
Interactions

EYA2 interacts with various proteins, including GNAI2 and GNAZ, which are involved in signal transduction pathways . These interactions highlight the multifunctional nature of EYA2 in cellular processes.

Research and Applications

Recombinant human EYA2 protein is widely used in research to study its function and role in various biological processes. It is often used in assays such as Western blot, ELISA, and protein arrays . However, it is important to note that recombinant EYA2 protein may contain endotoxins and is not suitable for use with live cells .

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