ErbB4 Human
HEK293 Cells.
Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4, 4ICD, E4ICD, s80HER4, ERBB4, HER4, receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1, receptor tyrosine-protein kinase erbB-4, tyrosine kinase-type cell surface receptor HER4, avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4, proto-oncogene-like protein c-ErbB-4, ALS19, v-erb-a erythroblastic leukemia viral oncogene homolog 4, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4, human epidermal growth factor receptor 4.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
ErbB4 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 863 amino acids (26-649 a.a) and having a molecular mass of 96.6 kDa.
ErbB4 is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
The epidermal growth factor receptor (EGFR) family, comprising receptor tyrosine kinases, plays a crucial role in mediating the effects of EGF family growth factors. ErbB4, a member of this family, functions as a receptor tyrosine kinase. Structurally, it exists as a single-pass type I transmembrane protein characterized by multiple furin-like cysteine-rich domains, a PDZ domain binding motif, a tyrosine kinase domain, and a phosphotidylinositol-3 kinase binding site. ErbB4 exhibits low affinity for its ligands and is found in various tissues including normal skeletal muscle, heart, pituitary, brain, and certain breast carcinomas. In its monomeric form, ErbB4 contributes to neuronal development, heart development, and cancer progression.
Recombinant Human ErbB4, expressed in HEK293 cells, is a single, glycosylated polypeptide chain. It consists of 863 amino acids, spanning from position 26 to 649, with a molecular weight of 96.6 kDa. The protein is engineered with a C-terminal fusion of a 239 amino acid hIgG-His-Tag and purified using proprietary chromatographic techniques.
Sterile filtered, colorless solution.
The ErbB4 solution is provided at a concentration of 0.25 mg/ml in phosphate buffered saline (pH 7.4) containing 10% glycerol.
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended periods, storage at -20°C in a frozen state is recommended. To enhance long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing should be avoided.
Purity is determined to be greater than 90.0% using SDS-PAGE analysis.
The biological activity of the protein is evaluated based on its binding affinity to Human NRG1 (CAT# cyt-1186) in a functional ELISA assay. The ED50 value is determined to be less than or equal to 0.2 ug/ml.
Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4, 4ICD, E4ICD, s80HER4, ERBB4, HER4, receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1, receptor tyrosine-protein kinase erbB-4, tyrosine kinase-type cell surface receptor HER4, avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4, proto-oncogene-like protein c-ErbB-4, ALS19, v-erb-a erythroblastic leukemia viral oncogene homolog 4, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4, human epidermal growth factor receptor 4.
HEK293 Cells.
QSVCAGTENK LSSLSDLEQQ YRALRKYYEN CEVVMGNLEI TSIEHNRDLS FLRSVREVTG YVLVALNQFR YLPLENLRII RGTKLYEDRY ALAIFLNYRK DGNFGLQELG LKNLTEILNG GVYVDQNKFL CYADTIHWQD IVRNPWPSNL TLVSTNGSSG CGRCHKSCTG RCWGPTENHC QTLTRTVCAE QCDGRCYGPY VSDCCHRECA GGCSGPKDTD CFACMNFNDS GACVTQCPQT FVYNPTTFQL EHNFNAKYTY GAFCVKKCPH NFVVDSSSCV RACPSSKMEV EENGIKMCKP CTDICPKACD GIGTGSLMSA QTVDSSNIDK FINCTKINGN LIFLVTGIHG DPYNAIEAID PEKLNVFRTV REITGFLNIQ SWPPNMTDFS VFSNLVTIGG RVLYSGLSLL ILKQQGITSL QFQSLKEISA GNIYITDNSN LCYYHTINWT TLFSTINQRI VIRDNRKAEN CTAEGMVCNH LCSSDGCWGP GPDQCLSCRR FSRGRICIES CNLYDGEFRE FENGSICVEC DPQCEKMEDG LLTCHGPGPD NCTKCSHFKD GPNCVEKCPD GLQGANSFIF KYADPDRECH PCHPNCTQGC NGPTSHDCIY YPWTGHSTLP QHARLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH
Product Science Overview
Tyrosine kinase ErbB-4, also known as HER4, is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This family includes four closely related receptors: EGFR (ErbB-1), ErbB-2 (HER2/neu), ErbB-3 (HER3), and ErbB-4 (HER4). These receptors play crucial roles in the regulation of cell growth, survival, differentiation, and migration.
ErbB-4 is a single-pass type I transmembrane protein with multiple functional domains . These include:
- Furin-like cysteine-rich domains: These domains are involved in ligand binding.
- Tyrosine kinase domain: This domain is responsible for the receptor’s kinase activity, which phosphorylates tyrosine residues on target proteins.
- Phosphatidylinositol-3 kinase (PI3K) binding site: This site is involved in the activation of the PI3K/AKT signaling pathway.
- PDZ domain binding motif: This motif is involved in protein-protein interactions.
ErbB-4 is activated by binding to specific ligands, including neuregulins (NRG-2, NRG-3, and NRG-4), heparin-binding EGF-like growth factor, and betacellulin . Upon ligand binding, ErbB-4 undergoes dimerization and autophosphorylation, leading to the activation of downstream signaling pathways that regulate various cellular processes, including mitogenesis and differentiation .
ErbB-4 plays a critical role in the development and function of various tissues, including the heart, nervous system, and mammary glands. It is involved in the regulation of cell proliferation, differentiation, and survival. Additionally, ErbB-4 signaling has been implicated in the development of the central nervous system and the regulation of synaptic plasticity .
Mutations and dysregulation of ErbB-4 have been associated with various diseases, including cancer and neurological disorders . For example:
- Cancer: Mutations in the ERBB4 gene have been identified in several types of cancer, including melanoma, lung adenocarcinoma, and medulloblastoma . These mutations can lead to aberrant signaling and uncontrolled cell growth.
- Neurological Disorders: Single-nucleotide polymorphisms (SNPs) in the ERBB4 gene have been linked to schizophrenia and familial amyotrophic lateral sclerosis (ALS) type 19 .
Human recombinant ErbB-4 is produced using recombinant DNA technology, which involves the insertion of the ERBB4 gene into a suitable expression system, such as bacteria, yeast, or mammalian cells. This allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant ErbB-4 is used in various studies to investigate its structure, function, and role in disease, as well as to develop potential therapeutic interventions targeting ErbB-4 signaling pathways.
