ErbB2 Human, Sf9
Receptor tyrosine-protein kinase erbB-2, EC 2.7.10.1, p185erbB2, C-erbB-2, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340 antigen, NEU, NGL, HER2, TKR1, HER-2, c-erb B2, HER-2/neu.
Greater than 85.0% as determined by SDS-PAGE.
Description
ErbB2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 638 amino acids (23-652) and having a molecular mass of 70.4kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). ErbB2 is fused to 8 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Receptor tyrosine-protein kinase erbB-2, EC 2.7.10.1, p185erbB2, C-erbB-2, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340 antigen, NEU, NGL, HER2, TKR1, HER-2, c-erb B2, HER-2/neu.
TQVCTGTDMK LRLPASPETH LDMLRHLYQG CQVVQGNLEL TYLPTNASLS FLQDIQEVQG YVLIAHNQVR QVPLQRLRIV RGTQLFEDNY ALAVLDNGDP LNNTTPVTGA SPGGLRELQL RSLTEILKGG VLIQRNPQLC YQDTILWKDI FHKNNQLALT LIDTNRSRAC HPCSPMCKGS RCWGESSEDC QSLTRTVCAG GCARCKGPLP TDCCHEQCAA GCTGPKHSDC LACLHFNHSG ICELHCPALV TYNTDTFESM PNPEGRYTFG ASCVTACPYN YLSTDVGSCT LVCPLHNQEV TAEDGTQRCE KCSKPCARVC YGLGMEHLRE VRAVTSANIQ EFAGCKKIFG SLAFLPESFD GDPASNTAPL QPEQLQVFET LEEITGYLYI SAWPDSLPDL SVFQNLQVIR GRILHNGAYS LTLQGLGISW LGLRSLRELG SGLALIHHNT HLCFVHTVPW DQLFRNPHQA LLHTANRPED ECVGEGLACH QLCARGHCWG PGPTQCVNCS QFLRGQECVE ECRVLQGLPR EYVNARHCLP CHPECQPQNG SVTCFGPEAD QCVACAHYKD PPFCVARCPS GVKPDLSYMP IWKFPDEEGA CQPCPINCTH SCVDLDDKGC PAEQRASPLT LEHHHHHH.
Product Science Overview
Tyrosine Kinase ErbB-2, also known as HER2 (Human Epidermal growth factor Receptor 2) or neu, is a member of the ErbB family of receptor tyrosine kinases. This protein plays a crucial role in the regulation of cell growth and differentiation. Overexpression or amplification of the ErbB-2 gene is associated with the development and progression of certain aggressive types of breast cancer and other cancers .
ErbB-2 is a 185-kDa transmembrane glycoprotein with intrinsic tyrosine kinase activity. It is unique among the ErbB family members because it has no known ligand and is constitutively active when overexpressed . The protein typically forms heterodimers with other ErbB family members, such as EGFR (Epidermal Growth Factor Receptor), to initiate downstream signaling pathways that promote cell proliferation and survival .
The recombinant form of ErbB-2 is produced using the baculovirus expression system in Sf9 insect cells. This system is advantageous for producing high yields of biologically active proteins with post-translational modifications similar to those in mammalian cells . The recombinant protein is often tagged with GST (Glutathione S-transferase) at the N-terminus to facilitate purification and detection .
ErbB-2 is a critical component of several cell surface receptor complexes and is essential for the regulation of peripheral microtubules. Upon activation, it triggers the MEMO1-RHOA-DIAPH1 signaling pathway, leading to the phosphorylation and inhibition of GSK3B at the cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing their association with the cell membrane and facilitating microtubule capture and stabilization .
The overexpression of ErbB-2 is a hallmark of certain types of breast cancer, making it a valuable target for therapeutic intervention. Drugs such as trastuzumab (Herceptin) have been developed to specifically target and inhibit ErbB-2, providing significant clinical benefits to patients with ErbB-2-positive cancers .
