ENTPD6 Mouse

Ectonucleoside Triphosphate Diphosphohydrolase 6 (ENTPD6), also known as NTPDase 6 or CD39L2, is a member of the ectonucleoside triphosphate diphosphohydrolase family. These enzymes are crucial in the regulation of extracellular nucleotide levels, which play significant roles in various physiological processes, including purinergic signaling, inflammation, and tissue repair .

ENTPD6 is a protein-coding gene that encodes an enzyme responsible for the hydrolysis of nucleoside triphosphates and diphosphates. This enzyme operates in a calcium- or magnesium-dependent manner and exhibits a strong preference for nucleoside diphosphates. It preferentially hydrolyzes GDP, IDP, and UDP, with slower hydrolysis of CDP, ITP, GTP, CTP, ADP, and UTP, and virtually no hydrolysis of ATP .

The enzyme contains four apyrase-conserved regions, which are characteristic of NTPases. These regions are essential for its catalytic activity. The membrane-bound form of ENTPD6 might support glycosylation reactions in the Golgi apparatus and, when released from cells, might catalyze the hydrolysis of extracellular nucleotides .

ENTPD6 plays a pivotal role in purinergic signaling by regulating the availability of extracellular nucleotides at purinergic P2 receptors. This regulation is crucial for various cellular functions, including signal transmission, inflammation control, and tissue repair .

The enzyme also generates extracellular nucleosides for cellular reuptake and salvage via nucleoside transporters of the plasma membrane. The extracellular adenosine formed acts as an agonist of purinergic P1 receptors. Additionally, ENTPD6 can produce and hydrolyze extracellular inorganic pyrophosphate, which is significant in controlling bone mineralization .

Mutations or dysregulation of ENTPD6 have been associated with several diseases, including Gaucher Disease Type IIIC and Endometrial Transitional Cell Carcinoma . Understanding the enzyme’s function and regulation can provide insights into potential therapeutic targets for these conditions.

Recombinant mouse ENTPD6 is widely used in research to study its biochemical properties, physiological roles, and potential therapeutic applications. The recombinant form allows for detailed investigations into the enzyme’s structure, function, and interactions with other molecules.