ENSA Human

Endosulfine Alpha Human Recombinant
Cat. No.
BT6715
Source
Escherichia Coli.
Synonyms
ARPP-19e, Alpha endosulfine isoform 3, Alpha-endosulfine, ENSA, MGC4319, MGC8394, MGC78563.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ENSA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 141 amino acids (1-121 a.a.) and having a molecular weight of 15.5kDa. The ENSA is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
ENSA, an endogenous sulfonylurea receptor ligand, diminishes K(ATP) channel currents by impeding sulfonylurea binding, consequently stimulating insulin secretion. As a member of the conserved cAMP-regulated phosphoprotein (ARPP) family, ENSA, or Endosulfine Alpha, acts as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. This function establishes it as an endogenous regulator of KATP channels, influencing insulin secretion through KATP channel interaction. ENSA's role in type 2 diabetes is currently under investigation. Its expression spans various tissues, including muscle, brain, and endocrine tissues.
Description
Recombinant human ENSA, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 141 amino acids (with residues 1-121 forming the ENSA sequence). It possesses a molecular weight of 15.5 kDa. The ENSA sequence is fused at the N-terminus to a 20 amino acid His-Tag. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The Endosulfine Alpha protein solution is buffered in 20mM Tris (pH 8.0) and supplemented with 1mM DTT and 10% glycerol.
Purity
Purity exceeds 90.0%, as determined by SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C is recommended. To ensure optimal stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Minimize freeze-thaw cycles to maintain product integrity.
Synonyms
ARPP-19e, Alpha endosulfine isoform 3, Alpha-endosulfine, ENSA, MGC4319, MGC8394, MGC78563.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSQKQEEENP AEETGEEKQD TQEKEGILPE RAEEAKLKAK YPSLGQKPGG SDFLMKRLQK GQKYFDSGDY NMAKAKMKNK QLPSAGPDKN LVTGDHIPTP QDLPQRKSSL VTSKLAGGQV E.

Product Science Overview

Gene and Protein Structure

The ENSA gene is located on chromosome 1q21.3 . The protein encoded by this gene plays a crucial role in various cellular processes. It is known to be an endogenous regulator of ATP-sensitive potassium (KATP) channels through its interaction with the beta-cell sulfonylurea receptor (SUR1) . The KATP channel is an octameric complex composed of two structurally unrelated types of subunits: Kir6.2 (KCNJ11) and the sulfonylurea receptor (SUR) .

Function and Mechanism

Endosulfine Alpha is involved in the regulation of insulin secretion. It modulates insulin release by inhibiting the binding of sulfonylureas to their receptor in vitro . This protein also inhibits cloned KATP channel currents, thereby stimulating insulin secretion . Additionally, it has been proposed as a candidate gene for type 2 diabetes due to its role in insulin regulation .

During mitosis, Endosulfine Alpha acts as a protein phosphatase inhibitor, specifically inhibiting protein phosphatase 2A (PP2A) . When phosphorylated at Ser-67, it interacts with PPP2R2D (PR55-delta) and inhibits its activity, which is essential for maintaining high cyclin-B1-CDK1 activity during the M phase .

Clinical Significance

Mutations or dysregulation of the ENSA gene have been associated with various diseases, including Charcot-Marie-Tooth Disease, Axonal, Type 2F, and Primary Ciliary Dyskinesia . The protein’s role in insulin secretion also links it to diabetes research and potential therapeutic targets .

Research and Applications

Recombinant human Endosulfine Alpha has been expressed in various systems for research purposes. It is used to study its role in insulin secretion, cell cycle regulation, and its potential as a therapeutic target for diabetes and other related disorders .

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