ENHO Human

Energy Homeostasis Associated Human Recombinant
Cat. No.
BT6589
Source
Escherichia Coli.
Synonyms
Adropin, Energy homeostasis-associated protein, ENHO, C9orf165, UNQ470.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

ENHO Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 34-76) containing 121 amino acids including extra 78 N-terminal amino acids. The total molecular mass is 13.05kDa (calculated).

Product Specs

Introduction
Energy Homeostasis Associated (ENHO) is involved in maintaining glucose balance and lipid metabolism. It is found in the liver and brain. Research is ongoing to understand its role in obesity and diabetes.
Description
Recombinant human ENHO, produced in E. coli, is a single, non-glycosylated polypeptide chain (amino acids 34-76) consisting of 121 amino acids, including an additional 78 N-terminal amino acids. The calculated molecular mass is 13.05 kDa.
Physical Appearance
White, lyophilized powder.
Formulation
ENHO was filtered at 0.4 μm and lyophilized from a 0.5 mg/mL solution in phosphate-buffered saline (PBS).
Solubility
To prepare a working solution, add deionized water to the lyophilized powder to achieve a concentration of approximately 0.5 mg/mL. Allow the pellet to dissolve completely. This product is not sterile. Filter the solution through a sterile filter before using it in cell culture.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the solution to avoid repeated freezing and thawing cycles. The reconstituted protein remains stable at 4°C for a limited period and shows no changes after two weeks at this temperature.
Purity
Purity is greater than 90%, as determined by SDS-PAGE.
Synonyms
Adropin, Energy homeostasis-associated protein, ENHO, C9orf165, UNQ470.
Source
Escherichia Coli.
Amino Acid Sequence
MGGKSNGEKK YIVGFKQGFK SCAKKEDVIS EKGGKLQKCF KYVDAASATL NEKAVEELKK DPSVAYVEED KLFKALTSCHSRSADVDSLS ESSPNSSPGP CPEKAPPPQK PSHEGSYLLQ P.

Product Science Overview

Background and Discovery

The concept of energy homeostasis has been studied for decades, with significant advancements in understanding the molecular mechanisms involved. The discovery of proteins and hormones that regulate energy balance has been pivotal in this field. One such protein is the human recombinant version of Energy Homeostasis Associated protein.

Structure and Function

Energy Homeostasis Associated (Human Recombinant) is a protein that plays a central role in regulating energy balance. It is produced using recombinant DNA technology, which involves inserting the gene encoding the protein into a host organism, such as bacteria or yeast, to produce the protein in large quantities.

This protein interacts with various receptors and signaling pathways in the body to regulate energy intake and expenditure. It is involved in processes such as:

  • Appetite Regulation: The protein influences the hypothalamus, a region of the brain that controls hunger and satiety. By modulating the activity of neurons in the hypothalamus, it helps regulate food intake.
  • Energy Expenditure: It affects metabolic rate and energy expenditure by influencing the activity of mitochondria, the energy-producing organelles in cells.
  • Glucose Homeostasis: The protein plays a role in maintaining blood glucose levels by affecting insulin sensitivity and glucose uptake in tissues.
Clinical Significance

The study of Energy Homeostasis Associated (Human Recombinant) has significant implications for understanding and treating metabolic disorders such as obesity and diabetes. By elucidating the mechanisms through which this protein regulates energy balance, researchers can develop targeted therapies to address these conditions.

For instance, recombinant versions of this protein can be used in research and clinical settings to study its effects on metabolism and to develop drugs that mimic or enhance its activity. This approach has the potential to provide new treatments for metabolic diseases that are currently difficult to manage.

Research and Future Directions

Ongoing research is focused on further understanding the molecular mechanisms through which Energy Homeostasis Associated (Human Recombinant) exerts its effects. Studies are exploring its interactions with other proteins and signaling pathways, as well as its role in different tissues and organs.

Future directions in this field include:

  • Therapeutic Development: Developing drugs that target the pathways regulated by this protein to treat metabolic disorders.
  • Biomarker Identification: Identifying biomarkers associated with the activity of this protein to diagnose and monitor metabolic diseases.
  • Personalized Medicine: Using knowledge of this protein’s function to develop personalized treatment plans for individuals with metabolic disorders.

In conclusion, Energy Homeostasis Associated (Human Recombinant) is a crucial protein in the regulation of energy balance. Its study provides valuable insights into the mechanisms of energy homeostasis and offers promising avenues for the development of new therapies for metabolic diseases.

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