EMG1 Human

EMG1 is a 244 amino acid protein that is localized to the nucleolus, a substructure within the nucleus of eukaryotic cells. The nucleolus is the site of ribosome synthesis and assembly, and EMG1 is involved in the processing of pre-18S rRNA, which is a component of the small ribosomal subunit .

The primary function of EMG1 is to participate in the assembly of the small ribosomal subunit. It is thought to control the methylation of rRNA during ribosome synthesis, which is a critical step in the formation of functional ribosomes. Ribosomes are essential for protein synthesis in all living cells, and any disruption in their assembly can lead to severe cellular dysfunction .

Mutations in the EMG1 gene have been associated with Bowen-Conradi syndrome, a rare genetic disorder characterized by severe growth retardation, microcephaly, and other developmental abnormalities. This highlights the importance of EMG1 in normal cellular function and development .

Recombinant human EMG1 protein is produced using Escherichia coli (E. coli) expression systems. The recombinant protein typically includes a His-tag at the N-terminus to facilitate purification. The protein is purified using conventional chromatography techniques and is available with a purity of over 95%, as determined by SDS-PAGE .

Recombinant EMG1 protein is used in various research applications, including studies on ribosome biogenesis, rRNA processing, and the effects of mutations on protein function. It is also used in structural biology to understand the detailed mechanisms of ribosome assembly and function .

Recombinant EMG1 protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain protein stability. The protein is typically supplied in a buffer containing Tris-HCl, NaCl, glycerol, and DTT to ensure its stability and activity .