ELANE Mouse
Sf9, Insect cells.
Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastase.
Greater than 90.0% as determined by SDS-PAGE.
Description
ELANE Mouse produced in Sf9 Insect cells is a single, glycosylated polypeptide chain (27-265a.a.) fused to a 6 aa His Tag at C-terminus containing 245 amino acids and having a molecular mass of 26.8kDa.
ELANE shows multiple bands between 28-40kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Elastase-2, also known as ELANE, is a serine protease of the chymotrypsin family. This enzyme plays a crucial role in breaking down elastin, a key component of connective tissue that provides elasticity. It works in conjunction with collagen to regulate the mechanical properties of these tissues. In neutrophils, a type of white blood cell, ELANE targets and degrades the Outer membrane protein A (OmpA) found in E. coli and other Gram-negative bacteria. The activity of ELANE is tightly regulated by alpha 1-antitrypsin (A1AT), an acute-phase protein. A1AT acts as a potent inhibitor by binding almost irreversibly to the active site of elastase, effectively preventing it from breaking down elastin. This inhibition is crucial because a deficiency in A1AT, often referred to as A1AD, can lead to the uncontrolled breakdown of elastic fiber by elastase, potentially resulting in various health complications.
This product consists of the recombinant mouse ELANE protein, produced in Sf9 insect cells. It is a single, glycosylated polypeptide chain that encompasses amino acids 27 to 265 from the original sequence. The protein also includes a 6 amino acid His Tag fused at the C-terminus for purification purposes. With a total of 245 amino acids, the recombinant ELANE protein has a molecular weight of 26.8 kDa. Under reducing conditions on SDS-PAGE, it appears as multiple bands between 28-40 kDa. The protein has been purified using proprietary chromatographic techniques to ensure high purity.
The ELANE protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and also contains 30% glycerol and 0.1M NaCl for stability.
To ensure product stability, store the vial at 4°C if it will be used within 2-4 weeks. For long-term storage exceeding this period, it is recommended to freeze the product at -20°C. To further enhance stability during prolonged storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%. Repeated freezing and thawing of the product should be avoided to maintain optimal product integrity.
The purity of ELANE Mouse is greater than 90%, as determined by SDS-PAGE analysis.
Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastase.
Sf9, Insect cells.
SEIVGGRPAR PHAWPFMASL QRRGGHFCGA TLIARNFVMS AAHCVNGLNF RSVQVVLGAH
DLRRQERTRQ TFSVQRIFEN GFDPSQLLND IVIIQLNGSA TINANVQVAQ LPAQGQGVGD
RTPCLAMGWG RLGTNRPSPS VLQELNVTVV TNMCRRRVNV CTLVPRRQAG ICFGDSGGPL
VCNNLVQGID SFIRGGCGSG LYPDAFAPVA EFADWINSII RSHNDHLLTH PKDREGRTNH HHHHH
Product Science Overview
Recombinant Mouse Neutrophil Elastase/ELA2 is derived from a mouse myeloma cell line, NS0. The protein sequence ranges from Ser27 to Arg260 and includes a C-terminal 10-His tag for purification purposes . The predicted molecular mass of the protein is approximately 27 kDa, but it appears as a 34 kDa band under reducing conditions in SDS-PAGE due to post-translational modifications .
Neutrophil Elastase/ELA2 is involved in various biological processes, including:
- Degradation of Extracellular Matrix: It breaks down elastin, collagen, and other extracellular matrix components, facilitating tissue remodeling and repair .
- Immune Response: ELA2 plays a role in the immune response by degrading bacterial virulence factors and modulating inflammation .
- Regulation of Inflammatory Processes: It helps regulate the activity of cytokines and chemokines, thus influencing the inflammatory response .
Recombinant Mouse Neutrophil Elastase/ELA2 is used in various research applications, including:
- Enzyme Activity Studies: To understand the enzyme kinetics and substrate specificity.
- Drug Development: As a target for developing inhibitors that can modulate its activity in diseases like chronic obstructive pulmonary disease (COPD) and cystic fibrosis .
- Inflammation Research: To study its role in inflammatory processes and immune response .
