EIF5A Antibody
Eukaryotic Translation Initiation Factor 5A, Mouse Anti Human
Cat. No.
BT16721
Source
Synonyms
EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, EIF5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-4D, Rev-binding factor.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Introduction
EIF5A is the only known protein containing the unusual amino acid hypusine, formed by the sequential action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as a substrate. This protein is involved in the first step of peptide bond formation in translation; however, further evidence suggests that it acts as a universally conserved translation elongation factor. EIF5A modulation is linked to cell proliferation and cancer development. Increased EIF5A expression is observed in the peripheral blood mononuclear cells (PBMCs) of HIV-1 infected individuals. EIF5A regulates crucial cellular processes, including cell survival and senescence, by influencing the stability of specific mRNAs. Heat stress can lead to a decrease in EIF5A levels in human pancreatic cancer cells, suggesting that EIF5A stability plays a role in determining cell fate following severe heat stress.
Formulation
The antibody is supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) at pH 7.4, containing 0.1% sodium azide as a preservative.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be stored at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles to maintain antibody stability.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and a shelf life of 1 month when stored at 4°C.
Applications
The EIF5A antibody has been validated for use in ELISA, Western blot, and Immunofluorescence assays to ensure its specificity and reactivity. However, it is essential to optimize the antibody concentration for each specific application to achieve optimal results. The recommended dilution range for Western blot and Immunofluorescence is 1:250 to 1:500. A starting dilution of 1:250 is recommended for initial experiments.
Synonyms
EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, EIF5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-4D, Rev-binding factor.
Purification Method
EIF5A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT2E9AT.
Immunogen
Anti-human EIF5A mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human EIF5A amino acids 1-154 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Introduction
Eukaryotic Translation Initiation Factor 5A (eIF5A) is a highly conserved protein found in eukaryotic cells and archaea. It plays a crucial role in the initiation of translation, a fundamental process in protein synthesis. eIF5A is unique due to its post-translational modification, known as hypusination, which is essential for its activity .
Discovery and Nomenclature
Structure and Conservation
eIF5A is a small, acidic protein with a molecular weight of approximately 17 kDa and consists of 157 amino acids. It is highly conserved across species, including plants, indicating its vital physiological role. This conservation suggests that eIF5A has been maintained throughout evolution due to its essential functions .
Function and Mechanism
eIF5A is involved in various cellular processes, including:
- Translation Elongation and Termination: eIF5A promotes translation elongation and termination, particularly in ribosome stalling at specific amino acid sequences. It binds between the exit (E) and peptidyl (P) sites of the ribosome, facilitating the rescue of stalled ribosomes .
- Hypusination: The unique post-translational modification of eIF5A, hypusination, involves the addition of a hypusine residue, which is critical for its function. This modification is catalyzed by deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH) .
- Cell Cycle Progression and Actin Dynamics: eIF5A is also implicated in cell cycle progression, actin dynamics, mRNA decay, and stress response pathways .
Physiological Roles and Clinical Implications
eIF5A has been studied extensively for its role in various physiological processes and diseases:
- Cancer: eIF5A is involved in the development and progression of cancers, particularly through its isoform A2, which is mainly expressed in cancer cells. In contrast, isoform A1 is ubiquitously expressed in normal cells .
- Ischemic Tolerance and Organ Transplantation: eIF5A’s role in ischemic tolerance and metabolic adaptation has opened up potential clinical applications, such as organ transplantation, myocardial infarction, and stroke .
- Genetic Diseases: Recent genomic and transcriptomic studies have revealed the involvement of eIF5A in genetic diseases, highlighting its potential as a therapeutic target .
Mouse Anti-Human eIF5A Antibodies
Mouse anti-human eIF5A antibodies are used in research to study the protein’s function and its role in various diseases. These antibodies are crucial for detecting and quantifying eIF5A in different experimental settings, aiding in the understanding of its physiological and pathological roles.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
