EIF4E Mouse
eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, Eif4e.
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Description
EIF4E Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 241 amino acids (1-217 a.a) and having a molecular mass of 27.6kDa. EIF4E is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, Eif4e.
MGSSHHHHHH SSGLVPRGSH MGSHMATVEP ETTPTTNPPP AEEEKTESNQ EVANPEHYIK HPLQNRWALW FFKNDKSKTW QANLRLISKF DTVEDFWALY NHIQLSSNLM PGCDYSLFKD GIEPMWEDEK NKRGGRWLIT LNKQQRRSDL DRFWLETLLC LIGESFDDYS DDVCGAVVNV RAKGDKIAIW TTECENRDAV THIGRVYKER LGLPPKIVIG YQSHADTATK SGSTTKNRFV V
Product Science Overview
Eukaryotic Translation Initiation Factor 4E (eIF4E) is a crucial protein involved in the initiation of translation in eukaryotic cells. It plays a significant role in recognizing the 7-methylguanosine cap structure at the 5’ end of messenger RNAs (mRNAs) and recruiting ribosomes to initiate protein synthesis . The recombinant mouse version of eIF4E is often used in research to study its function and regulatory mechanisms.
eIF4E is a component of the eukaryotic translation initiation factor 4F (eIF4F) complex, which also includes eIF4A and eIF4G . The primary function of eIF4E is to bind to the mRNA cap structure and facilitate the recruitment of the 43S preinitiation complex, which includes the small ribosomal subunit and other initiation factors . This process is essential for the initiation of cap-dependent translation, which is the predominant mechanism of translation initiation in eukaryotic cells.
The activity of eIF4E is tightly regulated and is considered a rate-limiting step in translation initiation . Dysregulation of eIF4E activity has been linked to various diseases, including cancer, neurodevelopmental disorders such as autism spectrum disorder (ASD), and Fragile X Syndrome (FXS) . The interaction between eIF4E and eIF4G is crucial for the assembly of the translational machinery, and this interaction is inhibited by 4E-binding proteins (4E-BPs), which compete for the same binding site on eIF4E .
Recombinant mouse eIF4E is a full-length protein expressed in Escherichia coli and is often tagged with a His tag for purification purposes . This recombinant protein is used in various biochemical and structural studies to understand the function and regulation of eIF4E. It is also used in assays to study the interaction between eIF4E and its binding partners, such as eIF4G and 4E-BPs .
Research on eIF4E has provided significant insights into the mechanisms of translation initiation and its regulation. Structural studies have revealed the interaction between eIF4E and its molecular partners, which has implications for the development of new therapeutic strategies . For example, designing molecules that inhibit the interaction between eIF4E and eIF4G could provide new avenues for the treatment of diseases associated with dysregulated translation .
