Eukaryotic Translation Initiation Factor 3K (eIF3K) is a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex, which plays a crucial role in the initiation phase of protein synthesis in eukaryotic cells. The eIF3 complex is essential for the assembly of the 43S pre-initiation complex and the recruitment of the 40S ribosomal subunit to the mRNA. eIF3K, also known as eIF3 subunit K, is one of the smaller subunits of this complex and is encoded by the EIF3K gene in humans.
eIF3K is a protein that consists of approximately 25 kDa and is characterized by its unique structural domains that facilitate its interaction with other subunits of the eIF3 complex. The primary function of eIF3K is to stabilize the eIF3 complex and assist in the recruitment of the 40S ribosomal subunit to the mRNA. This process is critical for the accurate and efficient initiation of translation, which is the first step in protein synthesis.
The eIF3 complex, including eIF3K, is involved in several key steps of translation initiation:
eIF3K is not only crucial for the initiation of translation but also plays a role in the regulation of gene expression. The proper functioning of eIF3K is essential for cellular homeostasis and the response to various cellular stresses. Dysregulation of eIF3K and other subunits of the eIF3 complex has been implicated in various diseases, including cancer, where abnormal protein synthesis can lead to uncontrolled cell growth and proliferation.
Human recombinant eIF3K is produced using recombinant DNA technology, where the EIF3K gene is cloned and expressed in a suitable host system, such as Escherichia coli or yeast. The recombinant protein is then purified to obtain a functional eIF3K subunit that can be used for various research and therapeutic applications. The availability of human recombinant eIF3K allows researchers to study its structure, function, and interactions in detail, providing insights into its role in translation initiation and its potential as a therapeutic target.