EIF3J Human

Eukaryotic Translation Initiation Factor 3J Human Recombinant
Cat. No.
BT4701
Source
Escherichia Coli.
Synonyms
Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit 1, eIF-3-alpha, eIF3 p35, EIF3J, EIF3S1, eIF3-alpha.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EIF3J Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (70-258) and having a molecular mass of 24kDa (Molecular weight on SDS-PAGE will appear higher).
EIF3J is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Eukaryotic translation initiation factor 3 subunit J (EIF3J) is a protein that is part of the EIF-3 complex. This complex plays a critical role in the beginning stages of protein synthesis in cells. Specifically, EIF3 helps bring together the messenger RNA (mRNA, the instructions for making a protein) with the ribosome (the protein-making machinery) and the initiator tRNA (which carries the first building block of the protein). EIF3J itself interacts with specific parts of the ribosome and helps regulate its binding to mRNA. This interaction is important for ensuring that protein synthesis starts at the correct place on the mRNA.
Description
This product is a highly purified form of the human EIF3J protein. It has been produced in a laboratory setting using E. coli bacteria. The protein is a single chain of 210 amino acids and includes a His-tag, which is a short sequence of amino acids that allows for easy purification. The protein is provided in a solution that is suitable for storage and use in various research applications.
Physical Appearance
Clear and colorless solution that has been sterilized by filtration.
Formulation
The EIF3J protein is provided in a solution at a concentration of 0.5 mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol, and 200mM NaCl. These components help maintain the stability and solubility of the protein.
Stability
To ensure the longest shelf life, store the EIF3J at -20°C. If you plan to use the entire vial within 2-4 weeks, you can store it at 4°C. Avoid repeatedly freezing and thawing the protein, as this can damage its structure. For long-term storage, it is recommended to add a carrier protein such as HSA or BSA at a concentration of 0.1%.
Purity
This product contains greater than 90% pure EIF3J protein as determined by SDS-PAGE, a widely used technique for separating and analyzing proteins based on their size.
Synonyms
Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit 1, eIF-3-alpha, eIF3 p35, EIF3J, EIF3S1, eIF3-alpha.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MKISEKKKIA EKIKEKERQQ KKRQEEIKKR LEEPEEPKVL TPEEQLADKL RLKKLQEESD LELAKETFGV NNAVYGIDAM NPSSRDDFTE FGKLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DLADYGGYDG GYVQDYEDFM.

Product Science Overview

Structure and Composition

The eIF3 complex is the largest of the eukaryotic initiation factors and is essential for the assembly of the 43S pre-initiation complex. The eIF3j subunit, in particular, is known for its role in modulating mRNA start codon selection . In humans, eIF3j is considered a labile subunit, meaning it can dissociate from the eIF3 complex under certain conditions .

Function and Mechanism

eIF3j has been implicated in several key processes during translation initiation and termination. It facilitates the loading of release factors into the ribosome, which is crucial for translation termination . Specifically, eIF3j stimulates peptidyl-tRNA hydrolysis induced by a complex of eukaryotic release factors, eRF1 and eRF3 . This activity is essential for the proper termination of translation and the release of the newly synthesized polypeptide chain.

Biological Significance

The regulation of protein synthesis in eukaryotes predominantly occurs during translation initiation. eIF3, including the eIF3j subunit, is a key regulator of this process. The complex’s ability to modulate start codon selection and facilitate translation termination highlights its importance in maintaining the fidelity and efficiency of protein synthesis .

Research and Applications

Research on eIF3j and the eIF3 complex has provided valuable insights into the molecular mechanisms of translation initiation and termination. Studies using model organisms like Neurospora crassa have shown that eIF3 from this fungus is structurally and compositionally similar to human eIF3, making it a useful system for probing the structure and function of human-like eIF3 in living cells .

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