EIF3I Human

Eukaryotic Translation Initiation Factor 3I Human Recombinant
Cat. No.
BT4541
Source
Escherichia Coli.
Synonyms
Eukaryotic Translation Initiation Factor 3, Subunit I, EIF3S2, Eukaryotic Translation Initiation Factor 3, Subunit 2 Beta, 36kDa, Eukaryotic Translation Initiation Factor 3 Subunit 2, TRIP-1, eIF-3-beta, EIF3 P36, TGF-Beta Receptor-Interacting Protein 1, PRO2242, eIF3-beta, eIF3-p36, Eukaryotic Translation Initiation Factor 3 Subunit I, Eukaryotic Translation Initiation Factor 3, Subunit 2 (Beta, 36kD), Predicted Protein Of HQ2242, TGFbeta Receptor-Interacting Protein 1, eIF3i.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EIF3I Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 348 amino acids (1-325aa) and having a molecular mass of 38.9kDa.
EIF3I is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Eukaryotic translation initiation factor 3, subunit I (EIF3I) is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex. This complex is crucial for various stages of protein synthesis initiation. EIF3I, as part of the eIF-3 complex, assists in connecting the 40S ribosome and recruiting other initiation factors like eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5. This assembly forms the 43S pre-initiation complex (43S PIC). The eIF-3 complex plays a vital role in mRNA recruitment to the 43S PIC and the subsequent scanning of the mRNA to identify the AUG start codon. Additionally, the eIF-3 complex is essential for disassembling and recycling post-termination ribosomal complexes, which helps prevent premature joining of the 40S and 60S ribosomal subunits before initiation. EIF3I has been associated with diseases such as clonorchiasis and tonsillitis.
Description
This product consists of EIF3I Human Recombinant, produced in E. coli. It is a single, non-glycosylated polypeptide chain with 348 amino acids (1-325aa) and a molecular weight of 38.9kDa. A 23 amino acid His-tag is fused to the N-terminus of the EIF3I protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear solution that has undergone sterile filtration.
Formulation
The APOBEC4 protein solution is provided at a concentration of 1mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M UREA, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of the EIF3I Human Recombinant is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Eukaryotic Translation Initiation Factor 3, Subunit I, EIF3S2, Eukaryotic Translation Initiation Factor 3, Subunit 2 Beta, 36kDa, Eukaryotic Translation Initiation Factor 3 Subunit 2, TRIP-1, eIF-3-beta, EIF3 P36, TGF-Beta Receptor-Interacting Protein 1, PRO2242, eIF3-beta, eIF3-p36, Eukaryotic Translation Initiation Factor 3 Subunit I, Eukaryotic Translation Initiation Factor 3, Subunit 2 (Beta, 36kD), Predicted Protein Of HQ2242, TGFbeta Receptor-Interacting Protein 1, eIF3i.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA

Product Science Overview

Introduction

Eukaryotic Translation Initiation Factor 3 (eIF3) is a multiprotein complex essential for the initiation phase of eukaryotic translation. It plays a crucial role in the formation of the preinitiation complex (PIC) and the recruitment of mRNA to the ribosome. Among its subunits, eIF3I is a significant component that contributes to the overall function of the eIF3 complex.

Structure and Composition

The eIF3 complex in humans consists of 13 nonidentical subunits (eIF3a-m) with a combined molecular weight of approximately 800 kDa, making it the largest translation initiation factor . The eIF3I subunit is a single, non-glycosylated polypeptide chain containing 348 amino acids and has a molecular mass of 38.9 kDa . It is often produced recombinantly in E. coli or Sf9 Baculovirus cells for research purposes .

Function

eIF3I, along with other subunits of the eIF3 complex, is involved in various steps of translation initiation. The eIF3 complex binds to the small ribosomal subunit (40S) and serves as a scaffold for several other initiation factors, including eIF1, eIF1A, eIF2, and eIF5 . This binding facilitates the formation of the 43S preinitiation complex (PIC), which is essential for the recruitment of mRNA and the scanning of the mRNA leader sequence for the start codon .

Role in Translation Initiation

The eIF3 complex, including eIF3I, plays a pivotal role in the initiation of translation by:

  1. Recruiting mRNA: eIF3 stimulates the recruitment of mRNA to the 43S PIC, enabling the scanning process for the start codon .
  2. Modulating Fidelity: eIF3 modulates the fidelity of start codon selection, ensuring that translation initiates at the correct site .
  3. Preventing Premature Association: eIF3 impairs the premature association of the 40S and 60S ribosomal subunits, thereby regulating the timing of ribosome assembly .
Research and Applications

Recombinant eIF3I is widely used in research to study the mechanisms of translation initiation and the role of individual subunits in this process. Structural studies, such as cryo-electron microscopy (cryo-EM), have provided insights into the interactions between eIF3I and other components of the translation machinery . Additionally, recombinant eIF3I is used to investigate its potential role in various diseases and to develop therapeutic interventions targeting translation initiation.

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EIF3I Human, Sf9
Eukaryotic Translation Initiation Factor 3I Human Recombinant, Sf9
Cat. No.
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Source
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