EIF3F Human

Eukaryotic Translation Initiation Factor 3F Human Recombinant

Cat. No.

BT4482

Source

Escherichia Coli.

Synonyms

Eukaryotic Translation Initiation Factor 3, Subunit F, EIF3S5 , Eukaryotic Translation Initiation Factor 3, Subunit 5 (Epsilon, 47kD), EC 3.4.19.12, Deubiquitinating Enzyme EIF3f, eIF3f, eIF-3-epsilon, EIF3 P47, Eukaryotic Translation Initiation Factor 3 Subunit 5, eIF3-p47, Eukaryotic Translation Initiation Factor 3, Subunit 5 Epsilon, 47kDa.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

EIF3F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-357 a.a) and having a molecular mass of 40kDa.
EIF3F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Eukaryotic Translation Initiation Factor 3F, also known as EIF3F, is a protein belonging to the eIF-3 subunit F family. It is a crucial part of the eukaryotic translation initiation factor 3 (eIF-3) complex, which plays a vital role in multiple steps of protein synthesis initiation. The eIF-3 complex interacts with the 40S ribosome and facilitates the assembly of other initiation factors like eIF-1, eIF-1A, eIF 2: GTP: methionyl-tRNAi, and eIF-5, forming the 43S preinitiation complex (43S PIC). This complex is essential for recruiting mRNA and scanning it for the AUG start codon. EIF3F, as part of the eIF-3 complex, is also involved in the disassembly and recycling of ribosomal complexes after termination, preventing premature re-association of ribosomal subunits before the next initiation cycle. Dysregulation or mutations in EIF3F have been linked to diseases such as intrahepatic cholangiocarcinoma and cholangiocarcinoma.

Description

This product consists of the human EIF3F protein recombinantly produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 380 amino acids, with the first 357 amino acids representing the EIF3F sequence and a 23 amino acid His-tag fused at the N-terminus. The molecular weight of the recombinant protein is 40kDa, and it undergoes purification using proprietary chromatographic techniques.

Physical Appearance

The product appears as a clear, colorless solution that has been sterilized by filtration.

Formulation

The EIF3F protein is supplied in a solution at a concentration of 1mg/ml. The solution buffer contains 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M urea.

Stability

For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided to maintain protein integrity.

Purity

The purity of the EIF3F protein is greater than 90%, as determined by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL.

Product Science Overview

Introduction

Eukaryotic Translation Initiation Factor 3F (eIF3f) is a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex, which plays a crucial role in the initiation phase of protein synthesis in eukaryotic cells. This complex is essential for both cap-dependent and cap-independent translation initiation, facilitating the assembly of the 43S pre-initiation complex (PIC) and the recruitment of other initiation factors.

Structure and Function

eIF3f is a multifunctional protein that interacts with various proteins involved in cellular processes such as protein synthesis, DNA repair, and viral mRNA editing. It is a component of the eIF3 complex, which associates with the 40S ribosome and aids in the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S PIC. This complex is essential for the proper positioning of ribosomes at the mRNA start codon, ensuring accurate translation initiation.

Biological Significance

eIF3f has been implicated in several critical cellular processes. It exhibits deubiquitinase activity, which is essential for regulating the activation of the Notch signaling pathway. The Notch pathway is highly conserved and plays a vital role in development, with its misregulation linked to various diseases. eIF3f’s deubiquitinase activity targets the activated Notch receptor, preventing its monoubiquitination and facilitating its processing by gamma-secretase.

In addition to its role in translation initiation and Notch signaling, eIF3f is involved in cell cycle regulation and proliferation. Its deregulation can compromise cell viability, highlighting its importance in maintaining cellular homeostasis.

Clinical Relevance

Mutations or alterations in the expression of eIF3f have been associated with several diseases. For instance, eIF3f has been implicated in intellectual developmental disorders and neurodevelopmental disorders with dysmorphic facies and distal limb anomalies. Understanding the function and regulation of eIF3f can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of targeted therapies.

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EIF3F Human

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