EIF1AX Human

Eukaryotic Translation Initiation Factor 1 X-linked Human Recombinant
Cat. No.
BT4126
Source
Escherichia Coli.
Synonyms
eukaryotic translation initiation factor 1A, X-linked, eIF-4C, EIF1A, EIF4C, eIF-1A, EIF1AP1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EIF1AX Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-144a.a.) and having a molecular wieght of 18.6kDa. EIF1AX is fused to 20a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
EIF1AX, a key eukaryotic translation initiation factor, is crucial for binding the 43S complex (composed of a 40S subunit, eIF2/GTP/Met-tRNAi, and eIF3) to the 5' end of capped RNA. This factor is vital for optimal protein biosynthesis rates. EIF1AX promotes the separation of ribosomes into subunits and stabilizes the attachment of the initiator Met-tRNA (I) to 40 S ribosomal subunits.
Description
Recombinant human EIF1AX, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 150 amino acids (residues 1-144a.a.) with a molecular weight of 18.6 kDa. A 20a.a. His-Tag is fused to the N-terminus of EIF1AX, which is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile solution without any color.
Formulation
The EIF1AX protein solution is provided at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 5mM DTT, 200mM NaCl, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeding 90.0% as determined by SDS-PAGE analysis.
Synonyms
eukaryotic translation initiation factor 1A, X-linked, eIF-4C, EIF1A, EIF4C, eIF-1A, EIF1AP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKAD VILKYNADEA RSLKAYGELP EHAKINETDT FGPGDDDEIQ FDDIGDDDED IDDI

Product Science Overview

Structure and Function

EIF1AX is a component of the 43S pre-initiation complex (43S PIC), which is essential for the initiation of translation. The 43S PIC binds to the mRNA cap-proximal region, scans the mRNA 5’-untranslated region, and locates the initiation codon . EIF1AX enhances the formation of the cap-proximal complex and, together with EIF1, facilitates scanning, start codon recognition, and the promotion of the assembly of the 48S complex at the initiation codon . This process is crucial for the accurate selection of the start codon and the initiation of protein synthesis.

After the start codon is located, EIF1AX, together with EIF5B, orients the initiator methionine-tRNA in a conformation that allows the joining of the 60S ribosomal subunit to form the 80S initiation complex . This step is vital for the proper assembly of the ribosome and the commencement of translation.

Biological Significance

EIF1AX is essential for the binding of the 43S complex (comprising a 40S subunit, eIF2/GTP/Met-tRNAi, and eIF3) to the 5’ end of capped RNA . This binding is a critical step in the initiation of translation, as it ensures that the ribosome is correctly positioned to start protein synthesis. The accurate initiation of translation is fundamental for the proper expression of genes and the production of functional proteins.

Clinical Relevance

Mutations in the EIF1AX gene have been associated with various diseases, including differentiated thyroid carcinoma and thrombocythemia 1 . These mutations can disrupt the normal function of the EIF1AX protein, leading to aberrant translation initiation and the development of disease. Understanding the role of EIF1AX in translation initiation can provide insights into the molecular mechanisms underlying these diseases and potentially lead to the development of targeted therapies.

Research and Applications

Recombinant EIF1AX protein is widely used in research to study the mechanisms of translation initiation and the role of EIF1AX in this process. By using human recombinant EIF1AX, researchers can investigate the protein’s function in a controlled environment and gain insights into its interactions with other components of the translation machinery.

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