EHF Human

EHF, like other members of the ETS family, contains a highly conserved DNA-binding domain known as the ETS domain. This domain is characterized by a winged helix-turn-helix structure that binds to DNA sequences with a central GGA(A/T) motif . The ETS domain is not only involved in DNA binding but also in protein-protein interactions, which are essential for the regulation of various cellular processes .

The primary function of EHF is to regulate the expression of genes involved in epithelial cell differentiation, proliferation, and homeostasis. It is particularly important in maintaining the integrity and function of epithelial tissues, such as the skin and the lining of the gastrointestinal tract .

EHF is essential for the post-natal homeostasis of the epidermis and colonic epithelium. Studies have shown that EHF functions as a tumor suppressor in the colon, where its deletion can enhance the development of adenomas, a type of benign tumor . In addition, EHF is highly expressed in the colonic epithelium, and its absence increases susceptibility to colitis, an inflammatory condition of the colon .

In the liver, EHF is expressed in cholangiocytes and hepatocytes, where it plays a role in liver fibrosis, a condition characterized by the excessive accumulation of extracellular matrix proteins . The expression of EHF in other cell types is relatively low, indicating its specific role in epithelial tissues .

Recombinant EHF is produced using recombinant DNA technology, which involves inserting the EHF gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the production of large quantities of EHF protein, which can be used for various research and therapeutic purposes.

Recombinant EHF is valuable in studying the molecular mechanisms underlying epithelial cell function and the role of EHF in disease. It can also be used in drug development to identify potential therapeutic targets for conditions such as cancer and inflammatory diseases.