EGLN3 Human

Egl Nine Homolog 3 Human Recombinant
Cat. No.
BT6208
Source
E.coli.
Synonyms
Egl nine homolog 3 (C. elegans), Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3, HIF-PH3, PHD3, egl nine-like protein 3 isoform, HIF prolyl hydroxylase 3, EC 1.14.11.29, HPH-1, HPH-3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

EGLN3 Human Recombinant produced in E. coli is a single polypeptide chain containing 263 amino acids (1-239) and having a molecular mass of 29.8 kDa.
EGLN3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
EGLN3, also known as Egl Nine Homolog 3, is a member of the EGLN family of prolyl hydroxylases. This enzyme plays a crucial role in the body's response to low oxygen levels (hypoxia) by regulating the activity of hypoxia-inducible factors (HIFs), specifically HIF-alpha subunits. EGLN3 catalyzes the hydroxylation of HIF-alpha, leading to its recognition by the von Hippel-Lindau (VHL) tumor suppressor complex and subsequent degradation. This process is essential for maintaining oxygen homeostasis within cells. EGLN3 is particularly significant in regulating HIF-2A, limiting its activation in hypoxic conditions. Its activity has been observed in various cell types, including cardiovascular cells and HeLa cells, where it contributes to cellular adaptation to low oxygen. Notably, EGLN3 also influences glucose metabolism by hydroxylating PKM2 under hypoxia, thereby regulating glycolysis. In normal oxygen conditions, it participates in regulating the stability of ADRB2 through hydroxylation. The activity of EGLN3 is inhibited by polynitrogen compounds, likely through interaction with iron ions (Fe2+).
Description
Recombinant human EGLN3, expressed in E. coli, is a single polypeptide chain with a molecular weight of 29.8 kDa. The protein consists of 263 amino acids, encompassing residues 1-239 of EGLN3 and an N-terminal His-tag of 24 amino acids. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The provided EGLN3 solution has a concentration of 0.25 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 300mM NaCl, 5mM DTT, 2mM EDTA, and 50% glycerol.
Stability
For optimal storage, maintain the EGLN3 solution at 4°C if the entire volume will be utilized within 2-4 weeks. For prolonged storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of EGLN3 is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Egl nine homolog 3 (C. elegans), Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3, HIF-PH3, PHD3, egl nine-like protein 3 isoform, HIF prolyl hydroxylase 3, EC 1.14.11.29, HPH-1, HPH-3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED

Product Science Overview

Structure and Function

EGLN3 is a 2-oxoglutarate-dependent dioxygenase that mediates the hydroxylation of proline residues in target proteins. This hydroxylation is essential for the regulation of the hypoxia-inducible factor (HIF) pathway. Under normoxic (normal oxygen) conditions, EGLN3 hydroxylates specific proline residues on HIF-α subunits, marking them for degradation via the von Hippel-Lindau (VHL) ubiquitination complex .

Role in Hypoxia

Under hypoxic (low oxygen) conditions, the activity of EGLN3 is attenuated, allowing HIF-α subunits to escape degradation. These stabilized HIF-α subunits translocate to the nucleus, where they dimerize with HIF-β and activate the transcription of genes involved in the adaptive response to hypoxia. This includes genes that promote angiogenesis, erythropoiesis, and glycolysis .

Clinical Significance

EGLN3 has been implicated in various physiological and pathological processes:

  • Cancer: Dysregulation of EGLN3 has been associated with several cancers, including renal cell carcinoma and pheochromocytoma .
  • Cardiomyocyte and Neuronal Apoptosis: EGLN3 regulates apoptosis in cardiomyocytes and neurons. In cardiomyocytes, it disrupts the BAX-BCL2 complex, inhibiting the anti-apoptotic effect of BCL2. In neurons, it has a proapoptotic effect, likely through the regulation of CASP3 activity .
  • High-Altitude Adaptation: Genetic variations in EGLN3 have been linked to high-altitude polycythemia (HAPC) in Tibetans, a condition characterized by excessive erythrocytosis due to chronic hypoxia .
Research and Applications

Recombinant human EGLN3 is used in research to study its role in hypoxia signaling and its potential as a therapeutic target. Understanding the mechanisms by which EGLN3 regulates HIF and other pathways can provide insights into the development of treatments for diseases related to hypoxia and apoptosis.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.