EGFP Antibody
Green fluorescent protein, GFP.
Description
Product Specs
Green fluorescent protein, GFP.
EGFP antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Mouse Anti Monoclonal.
PAT1D9AT.
Anti EGFP mAb 1-239 amino acid is purified from E. coli.
Mouse IgG2a heavy chain and κ light chain.
Product Science Overview
Enhanced Green Fluorescent Protein (eGFP) is a widely used fluorescent marker in molecular and cellular biology. Derived from the original Green Fluorescent Protein (GFP) found in the jellyfish Aequorea victoria, eGFP has been modified to exhibit brighter fluorescence and greater stability. Mouse anti-GFP antibodies are commonly used to detect GFP and its variants in various experimental applications, including Western blots, immunohistochemistry, and flow cytometry.
GFP was first isolated in the 1960s, and its gene was cloned in the early 1990s. The discovery of GFP revolutionized biological imaging, allowing scientists to visualize and track proteins, cells, and entire organisms in real-time. eGFP is a mutant form of GFP that has been optimized for enhanced fluorescence and stability. It emits bright green light when exposed to blue or ultraviolet light, making it an ideal marker for live-cell imaging.
eGFP has been incorporated into various transgenic organisms, including mice, to study gene expression, protein localization, and cellular dynamics. For example, the ChAT-eGFP transgenic mouse line expresses eGFP under the control of the choline acetyltransferase (ChAT) promoter, allowing researchers to visualize cholinergic neurons in the central and peripheral nervous systems .
Mouse anti-GFP antibodies are monoclonal antibodies specifically designed to bind to GFP and its variants, including eGFP. These antibodies are highly specific and produce a strong signal with minimal background noise, making them valuable tools for detecting GFP-tagged proteins in various experimental setups .
- Western Blotting: Mouse anti-GFP antibodies are used to detect GFP-tagged proteins in Western blot assays. The antibody binds to the GFP tag, allowing researchers to visualize the protein of interest on a membrane.
- Immunohistochemistry: In immunohistochemical staining, mouse anti-GFP antibodies are used to detect GFP-expressing cells in tissue sections. This technique is particularly useful for studying the distribution and localization of GFP-tagged proteins in different tissues.
- Flow Cytometry: Mouse anti-GFP antibodies can be used in flow cytometry to analyze the expression of GFP-tagged proteins in individual cells. This application is valuable for studying cell populations and their characteristics.
